International Journal of Transplantation Research and Medicine provides a forum of information on the latest advances and new findings in the field of transplantation and medicine. This journal covers varied specializations of transplantation which under open access category provides the readers with wealth of information.

International Journal of Transplantation Research and Medicine is a peer-reviewed open access journal that ensures the faster publication of high quality articles. Two independent review comments followed by editor's decision will be considered to publish the article. IJTRM under open access category provides its global readers with free access to its content, thus serving the scientific community.

 
Journal Information

Title: International Journal of Transplantation Research and Medicine

ISSN: 2572-4045

Editor-in-chief: Lydia M Petrovic

NLM title abbreviation: Int J Transplant Res Med

ISO abbreviation: Int J Transplant Res Med

Other titles: IJTRM

Category: Surgery and Procedures

DOI: 10.23937/2572-4045

Peer review: Double blind

Review speed: 3 weeks

Fast-track review: 10 days

Publication format (s): Electronic and print

Publication policy: Open Access; COPE guide

Publication type(s): Periodicals

Publisher: ClinMed International Library

Country of publication: USA

Language: English

Contact email: contact@clinmedjournals.org

 
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 Open Access DOI:10.23937/2572-4045.1510036

Early Acute Renal Injury and Brain Dead

Kada AY, Kastali M and Bouyoucef KA

Article Type: Original Article | First Published: May 25, 2018

Brain death is the cause of a major systemic inflammatory response syndrome (SIRS) affecting all organs and responsible for part of their primary dysfunction and their lower survival after transplantation. Kidney failure (AKI: Acute Kidney Injury) is one of those consequences, it may be the cause of post-transplant complications if it is not supported quickly....
 

 Open Access DOI:10.23937/2572-4045.1510035

Ureteric Stricture following Pediatric Dual En-Bloc Renal Transplantation

Phillips BL, Forman CJ and Banga NR

Article Type: Case Report | First Published: May 25, 2018

This is a case report examining a urine leak and ureteric stricture following pediatric dual en-bloc renal transplantation. Despite intraoperative ureteric stenting, and post-operative nephrostomy, urinary leak continued. Both grafts grew in size from 5 cm at implantation, to 9.5 cm within 3 months. Ureteric reconstruction would expose the patient to unacceptable risk of losing both grafts, due to the close proximity of the ureters....
 

 Open Access DOI:10.23937/2572-4045.1510034

Transplantation of Decellularized Venous Valvular Grafts in an Ovine Model

Alexandru Mogaldea, Karolina Theodoridis, Klaus Hoffler, Axel Haverich, Andres Hilfiker and Serghei Cebotari

Article Type: Research Article | First Published: August 31, 2017

The surgical treatment of end-stage chronic venous insufficiency involves valvular repair or transplantation to restore venous valve function and structure. Current valve substitutes can have issues with durability, thrombogenicity, susceptibility to infection, and a lack of growth potential. Therefore, the development of tissue-engineered venous valves represents a promising solution....
 

 Open Access DOI:10.23937/2572-4045.1510033

Impact of Conditioning Including Anti-Thymocyte Globulin on Engraftment Kinetics and GvHD after Matched Related Allogeneic Stem Cell Transplantation

Olga Blau, Axel Nogai, Kathrin Rieger, Lutz Uharek, Antonio Pezzutto, Eckard Thiel and Igor Wolfgang Blau

Article Type: Research Article | First Published: August 12, 2017

Graft-Versus-Host Disease (GvHD) remains a major complication of Allogeneic Stem Cell Transplantation (alloSCT). Anti-Thymocyte Globulin (ATG), however, reduces the incidence and severity of GvHD after alloSCT. A small number of studies suggest a role for ATG in Match-Related Donor (MRD) alloSCT. The aim of this study was to assess the risk of acute and chronic GvHD, engraftment, survival, and mortality in patients who received ATG prior to MRD a...
 

 Open Access DOI:10.23937/2572-4045.1510032

Treatment of Coronary Artery Fistula Post-Cardiac Transplantation with Covered Stent: A Case Study and Review of Literature

Yip AMC, Chong AY, Stadnick E, Davies RA, Haddad H, Mielniczuk LM and Sharon Chih

Article Type: LITERATURE REVIEW | First Published: August 11, 2017

The majority of coronary artery fistulas are congenital and discovered incidentally on coronary angiography. Coronary artery fistulas may also develop after cardiac transplantation as a complication of endomyocardial biopsies. The natural history and optimal management of post-transplant coronary artery fistulas are uncertain....
 

 Open Access DOI:10.23937/2572-4045.1510031

Sustained In Vivo Perfusion of a Re-Endothelialized Tissue Engineered Porcine Liver

Shennen A Mao, Jaime M Glorioso, Faysal M Elgilani, Silvana B De Lorenzo, Michael C Deeds, Dominique Seetapun, Brian Perrault, Bruce P Amiot, Anisha Singh, Clara T Nicolas, Yujia Wang, E Heidi Cheek, Taofic Mounajjed, Allan B Dietz, Jeff J Ross and Scott L Nyberg

Article Type: Research Article | First Published: June 12, 2017

Liver transplantation remains the only definitive treatment for end-stage liver disease. With only 6000 donor livers available each year nationally, the need far exceeds the supply. Innovative cell-based therapies, such as hepatic tissue engineering, offer alternatives to supplement the limited supply of quality organs for transplantation....
 

 Open Access DOI:10.23937/2572-4045.1510030

Mycotic Aneurism in Renal Transplantation Associated to Persistent Leucopenia. Case Report and Review of the Literature

Lorena Noriega-Salas and Sinhue Escalona-Delgado

Article Type: Case Report | First Published: May 31, 2017

A 27-years-old female with end stage kidney disease, received a deceased donor kidney transplant, the induction immunosuppression consisted in polyclonal anti-lymphocyte therapy. The maintenance therapy included plus to the immunosuppressive drugs, valganciclovir as prophylaxis of CMV....
 

 Open Access DOI:10.23937/2572-4045.1510029

Strategies in Preventing Diabetes after Pancreatectomy Using Islet Auto- and Allo-Transplantation

Tori Tucker, Kevin Labadie, Christopher Kopan, Michael Alexander and Jonathan RT Lakey

Article Type: Research Article | First Published: May 15, 2017

Over the years, pancreatectomy has been the primary treatment for chronic pancreatitis. Since the 1970s, total pancreatectomy has been shown to be effective at relieving pain that was experienced due to chronic pancreatitis. However, total pancreatectomy alone has the significant side effect of post-surgical diabetes....
 

 Open Access DOI:10.23937/2572-4045.1510028

Lung Transplantation for Chronic Obstructive Pulmonary Disease: Outcomes before and after Implementation of the UNOS Lung Allocation Scoring System

Walker A Julliard, Keith C Meyer, Satoru Osaki, James D Maloney, Richard D Cornwell, David A Sonetti, Mehgan Holland and Nilto C De Oliveira

Article Type: Research Article | First Published: May 08, 2017

The United Network for Organ Sharing (UNOS) Lung Allocation Score (LAS) adopted in April 2005 to prioritize lung transplantation for patients at high risk of dying without a transplant may adversely affect outcomes for patients transplanted for Chronic Obstructive Pulmonary Disease (COPD)....
 

 Open Access DOI:10.23937/2572-4045.1510027

Distinctive CD8 T Cell Infiltration and Paucity of Regulatory T Cells in Chronic Graft-Versus-Host-Disease Lesions

Maria Claudia Rodrigues Moreira, Adriana Bonomo, Suzana A Pinheiro-da-Silva, Andrea Cordovil Pires, Rômulo Gonçalves Galvani, Daniela de Oliveira Pinto, Eliana Abdelhay and Maria Isabel Doria Rossi

Article Type: Research Article | First Published: April 15, 2017

Alterations in immune reconstitution have been implicated with the development of chronic graft-versus-host disease (cGVHD) in allogeneic bone marrow transplantation (BMT) recipients. Since T cells are important mediators in this disease, the aim of this study was to search for a biologic marker for cGVHD moderate and severe forms....
 

 Open Access DOI:10.23937/2572-4045.1510026

Review of Bacterial Urinary Tract Infection in Kidney Transplant Recipients: Incidence, Risk Factors and Impact on the Graft Survival

Fontsere S, Chacon N and Cordero E

Article Type: Review Article | First Published: April 13, 2017

Infectious complications remain a major cause of morbidity and mortality among transplant recipients. Urinary tract infection (UTI) is the most common infectious complication in kidney transplant recipients with a reported incidence of between 25%-75%. This varies widely likely due to differences in definition, diagnostic criteria, study design, and length of observation....
 

 Open Access DOI:10.23937/2572-4045.1510025

Disseminated Ochroconis in a Lung Transplant Recipient

Supriya Bhat, Maria Bembi, Sivagini Ganesh and Emily Blodget

Article Type: Case Report | First Published: March 29, 2017

Ochroconis is a darkly pigmented "black mold", which is thermotolerant and found in soil as well as in decaying vegetables, hot springs, cave rocks and Paleolithic paintings. It is known to cause encephalitis in domestic poultry, but there are increasing reports of it being a human pathogen....
 

 Open Access DOI:10.23937/2572-4045.1510024

Case Report and Review of the Literature: Resolution of Lithium-Induced Nephrogenic Diabetes Insipidus with Pre-Emptive Living Related Kidney Transplantation for End-Stage Renal Disease

B Daniel Campos, Natalia Velez-Ramos, Stephanie M Smith, Colin Lenihan and Marc L Melcher

Article Type: Case Report | First Published: March 09, 2017

Long-term lithium therapy is known to cause renal dysfunction, including nephrogenic diabetes insipidus (nDI) and chronic tubulointerstitial nephropathy, which may progress to end-stage renal disease (ESRD) in approximately 1% of patients. We report a case of resolution of lithium-induced nDI following living related kidney transplantation for ESRD secondary to chronic lithium toxicity....
 

 Open Access DOI:10.23937/2572-4045.1510023

B-Cell Targets to Treat Antibody-Mediated Rejection in Transplantation

Manuel Muro, Santiago Llorente, Jose A Galian, Francisco Boix, Jorge Eguia, Gema Gonzalez-Martinez, Maria R Moya-Quiles and Alfredo Minguela

Article Type: Commentary | First Published: October 22, 2016

Antibody-mediated rejection (AMR) in allograft transplantation can be defined with a rapid increase in the levels of specific serological parameters after organ transplantation, presence of donor specific antibodies (DSAs) against human leukocyte antigen (HLA) molecules, blood group (ABO) antigens and/or endothelial cell antigens (e.g. MICA, ECA, Vimentin, or ETAR) and also particular histological parameters....
 

 Open Access DOI:10.23937/2572-4045.1510022

Complications after Heart Transplantation: Hope for the Best, but Prepare for the Worst

Ana Carolina Alba, Eva Bain, Nicholas Ng, Madeleine Stein, Kathleen O'Brien, Farid Foroutan and Heather Ross

Article Type: Review Article | First Published: October 10, 2016

For selected patients living with the most advanced stages of heart failure, cardiac transplantation is considered the gold-standard treatment, providing substantial improvements in survival and quality of life. However, heart transplantation is not without risk, and almost all transplant recipients will suffer some form of complication, from mild to potentially fatal,and should be informed of this prior to transplant....
 

 Open Access DOI:10.23937/2572-4045.1510021

Anonymous Altruistic Living Kidney Donation in the US: Reality and Practice

Abby S Kazley, Raj Amarnath, Arun Palanisamy, David Taber, Kenneth Chavin, Prabhakar Baliga and Wendy Balliet

Article Type: Research Article | First Published: September 21, 2016

Living kidney donors have emerged as the best option to overcome the severe shortage of transplantable kidneys. A growing number of these living donor kidneys come from anonymous altruistic donors who are not related to the recipients according to UNOS data. This study examines the process of anonymous altruistic kidney donation and identifies barriers and variance in transplant center practices. Using a mock patient caller, 73 transplant centers...
 

 Open Access DOI:10.23937/2572-4045.1510020

Donation Over Age 70 and Older İs an Obstacle for Kidney Transplantation?

Ethem Unal and Aylin Acar

Article Type: Editorial Letter | First Published: July 17, 2016

he profound organ shortage has resulted in longer waiting times and increased mortality for those awaiting kidney transplantation. Consequently, patients are turning to older living donors. It is unclear if an upper age limit for donation should exist, both in terms of recipient and donor outcomes. However, there is a fact that surgery can present additional risks for older patients; this requires physicians to be especially careful about who the...
 

 Open Access DOI:10.23937/2572-4045.1510019

Influence of Metronidazole on Oral Busulfan Test Dose

Francine Attie de Castro, Vera Lucia Lanchote and Belinda Pinto Simoes

Article Type: Case Report | First Published: June 30, 2016

Individualization of busulfan (BU) dosing during pre-transplant conditioning for hematopoietic stem cell transplantation (HSCT) has been performed worldwide for more than a decade. The pharmacokinetic parameters of BU show high variability and studies report that high plasma concentrations (> 900 ng/mL) are associated with sinusoidal obstructive syndrome (SOS), whereas low plasma concentrations (< 600 ng/mL) are associated with graft rejection an...
 

 Open Access DOI:10.23937/2572-4045.1510018

Pharmacological Conditioning of Brain Dead Donor Hearts with Erythropoietin and Glyceryl Trinitrate: Clinical Experience

Gayathri Kumarasinghe, Arjun Iyer, Mark Hicks, Alasdair Watson, Hong Chew, Ling Gao, Jeanette Villanueva, Andrew Jabbour, Eugene Kotlyar, Christopher S Hayward, Anne M Keogh, Emily Granger, Paul C Jansz, Kumud K Dhital, Peter S Macdonald and Phillip Spratt

Article Type: Review Article | First Published: April 01, 2016

With the increasing success of heart transplantation, older and higher-risk donors and recipients are being accepted for transplantation. The risk of primary graft dysfunction (PGD) is thus increased. We investigated a 'pharmacological conditioning' strategy, where Celsior preservation solution supplemented with glyceryl trinitrate (GTN) and erythropoietin (EPO) was used for cardioplegia and hypothermic storage, and determined graft recovery and ...
 

 Open Access DOI:10.23937/2572-4045.1510017

Skin Lesions after Kidney Transplantation: An updated Review Including Recent Rare Cases

Ethem Unal

Article Type: Review Article | First Published: February 28, 2016

Skin disease is a significant cause of morbidity in chronically immunosuppressed patients, including organ transplant recipients. Cutaneous neoplasms are much more common in renal transplant recipients than in the general population, and are the most common malignancies in these patients. Better surgical techniques and recent advances in immunosuppressive therapy allow patients to survive for many years, free from any complications due to rejecti...
 

 Open Access DOI:10.23937/2572-4045.1510016

Blockade of ASC but not NLRP3 Inhibits DC Proliferation and T cell Activation in Response to Alloantigen

Sashi G Kasmisetty, Alana Shigeoka, Reza Elahimehr, Andrew Scheinok and Dianne B McKay

Article Type: Research Article | First Published: January 16, 2016

The NLRP3 inflammasome is a multimeric protein complex consisting of the sensor molecule NACHT, LRR and PYD domains-containing protein 3 (NLRP3), the adaptor molecule Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and caspase-1. Molecules with conserved molecular motifs trigger the assembly of the inflammasome and lead to maturation of pro-inflammatory cytokines that induce a mature inflammatory response. Em...
 

 Open Access DOI:10.23937/2572-4045.1510015

Total Pancreatectomy and Islet Auto-Transplantation in Children with Chronic Pancreatitis

Megan Berger, Melena Bellin, Sarah Jane Schwarzenberg and Srinath Chinnakotla

Article Type: Review Article | First Published: December 19, 2015

Chronic pancreatitis (CP) is an uncommon diagnosis in children, with an estimated incidence of fewer than 0.5 cases per 100,000 in patients younger than 25 years. Chronic pancreatitis in children most commonly results from genetic mutations, including mutations in the PRSS1, SPINK1, and CFTR genes. A recent multinational cross-sectional study of children with chronic pancreatitis demonstrated genetic etiology in upwards of 67% of cases while obst...
 

 Open Access DOI:10.23937/2572-4045.1510014

Total Pancreatectomy and Islet Auto-Transplantation for Chronic Painful Pancreatitis: An Overview

Megan Berger, Gregory J Beilman, Ty Dunn, David Sutherland and Srinath Chinnakotla

Article Type: Review Article | First Published: December 02, 2015

Chronic pancreatitis is a progressive and irreversible process that can lead to pain, pancreatic dysfunction, and even malignancy. Management can be difficult, with some patients proving refractory to standard medical or endoscopic treatments. These patients generally depend on narcotics to manage their symptoms. Total pancreatectomy and Islet Auto-Transplantation (TP-IAT) can offer relief for such patients by removing the root cause of their pai...
 

 Open Access DOI:10.23937/2572-4045.1510013

The Impact of Renal Transplantation on Lower Limb Perfusion

Paul Alexander, Emma Aitken, David Mansouri and David Kingsmore

Article Type: Research Article | First Published: November 25, 2015

The benefits of renal transplantation are now more accessible to older, more comorbid patients. Currently, those with peripheral vascular disease are often excluded partly due to the theoretical risk of post transplantation limb ischemia, where blood is diverted away from an already under-perfused leg. This study aims to determine if renal transplantation affects lower limb perfusion....
 

 Open Access DOI:10.23937/2572-4045.1510012

Infection after Pediatric Living Related Liver Transplantation: Timing, Types and Risk Factors

Behairy El-Sayed Behairy, Hatem Abdel-Sattar Konsowa, Haidy Mohammed Zakaria, Osama Hegazy Abd-Elsalam and Mostafa Mohamed Sira

Article Type: Research Article | First Published: November 16, 2015

The study was a retrospective cohort. It included 27 pediatric patients underwent liver transplantation (LTx). All patients' records were reviewed. A wide range of potential risk factors for infection and post-transplant complications were recorded. Follow-up data were collected for 1.5 years post-transplant, every infection attack during this period were subjected to detailed risk analysis....
 

 Open Access DOI:10.23937/2572-4045.1510011

Management of Bipolar Disorder in Liver Transplantation: A Single Center Experience

Humberto C Gonzalez, Paige E Morgan, Sheila Jowsey, Laura J Myhre, Adriana Vasquez and Kymberly D Watt

Article Type: Research Article | First Published: November 09, 2015

Bipolar disorder and end stage liver disease commonly intersect and their management may include evaluation for liver transplantation. Psychiatric illness may impair adherence to medical regimens jeopardizing the allograft, thus, generating divided opinions by liver transplant selection committees whether transplantation is even possible in this scenario. There is very limited data about the optimal management of bipolar disorder before and after...
 

 Open Access DOI:10.23937/2572-4045.1510010

A Complicated Case of Transplant Renal Artery Stenosis: A Case Report and Literature Review

Gregor Mlinsek, Alexander Jerman, Damjan Kovac, Jelka Lindic, Miro Mihelic, Nikola Lakic, Aljosa Kandus and Jernej Pajek

Article Type: Case Report | First Published: October 22, 2015

Transplant renal artery stenosis (TRAS) is a well recognized complication of kidney transplantation. Hypertension with or without an increasing creatinine level is the most common presentation. Recognition of TRAS is important because it represents a potentially reversible cause of hypertension, allograft loss and adverse patient outcome. Here we report a demanding case of TRAS in a kidney transplant recipient who received a pediatric kidney. The...
 

 Open Access DOI:10.23937/2572-4045.1510009

Could Accidental Ischemic Attacks Protect Against Subsequent Ischemic Reperfusion Injury?

Mohamed S. A. Mohamed

Article Type: Retrospective Study | First Published: August 20, 2015

Heart failure is the end result of various cardiac diseases that might be of ischemic or non-ischemic etiology. Implantation of ventricular assisting devices has been introduced as a bridge to heart transplantation, when heart failure reaches a critical degree of decompensation. The ischemic background of heart failure was suggested to have a protective effect in comparison to the non-ischemic background, based on the principles of ischemic condi...
 

 Open Access DOI:10.23937/2572-4045.1510008

Stem Cell Transplantation to Treat Severe Refractory Multiple Sclerosis

Athanasia Mouzaki, Marios I Garyfallou and Kokona Chatzantoni

Article Type: Review Article | First Published: August 13, 2015

In severe, drug-resistant Multiple Sclerosis (MS), Stem Cell Transplantation (SCT) was introduced as a treatment option about 20 years ago. Application of autologous SCT is based on the assumption that MS is not inherited in a Mendelian fashion and the auto reactive immune system can be replaced by a healthy one. Allogeneic SCT ensures that all leukocytes are of donor origin, but is applied rarely. Both approaches are fraught with problems result...
 

 Open Access DOI:10.23937/2572-4045.1510007

Impact of Donor Cytomegalovirus Serology and duration of Prophylaxis on Follow-Up Strategy in Lung Transplant Recipients

Jose M. Borro, Maria Delgado, Teresa Rey and Pablo Rama

Article Type: Original Article | First Published: July 04, 2015

Cytomegalovirus (CMV) infection is the most prevalent opportunistic infection after lung transplantation. It is reported in between 20% and 50% of cases after discontinuation of prophylaxis, depending on the series. CMV usually remains dormant in the lymphatic system, and its reactivation, due to immunosuppressive therapy, may have considerable adverse consequences in the immunocompromised host. Its many and varied effects include inflammation, i...
 

 Open Access DOI:10.23937/2572-4045.1510006

Incidental Discovery of a Donor-Derived Urothelial Carcinoma Following Kidney Transplantation

Kathryn Milks, Sriyesh Krishnan, Melanie Caserta, Robert J Stratta and Hisham Tchelepi

Article Type: Case Report | First Published: June 20, 2015

Urothelial carcinoma in renal transplant patients has been well described, particularly in the Asian literature, owing to the higher incidence of the disease in this part of the world, both in the native urothelial system and in the transplanted kidney. In the United States, however, urothelial carcinoma after kidney transplant is quite rare in the native urothelial system and extraordinarily rare in the renal allograft. It has been proposed in a...
 

 Open Access DOI:10.23937/2572-4045.1510005

Immunogenicity of Shared Molecular Epitopes in the Humoral Response as Biomarker of the Rupture of Immune Tolerance in Transplantation

Manuel Muro, Francisco Boix, Jorge Eguia, Maria R. Moya-Quiles, Gema GonzalezMartinez and Santiago Llorente

Article Type: Editorial | First Published: March 06, 2015

Th true role of donor-specific human leukocyte antigen (HLA) antibody (DSA) response to a solid allograft is not fully understood. Recently, new serum-screening assays (mainly based in the luminex technology) have strongly increased and defined the detection and specificity analysis of DSA in allosensitized patients in waiting list of transplantation or in transplanted recipients....
 

 Open Access DOI:10.23937/2572-4045.1510004

Simultaneous Liver Kidney Transplantation

Kirk B. Russ and Ashwani K. Singal

Article Type: Editorial | First Published: March 02, 2015

Since the introduction of model for end-stage liver disease (MELD) score in 2002 for listing patients for liver transplantation, frequency and proportion of simultaneous liver kidney (SLK) transplantation has increased by over 300%. This is mainly due to incorporation of renal function and serum creatinine as one of the major factors in determining the MELD score. ...
 

 Open Access DOI:10.23937/2572-4045.1510003

A Letter of Reminder to all Nephrologists and Surgeons: The importance of Sticking to KDOQI Guidelines for Selection and Placement of Hemodialysis Access

Ethem Unal

Article Type: Editorial | First Published: February 26, 2015

Kidney Disease Outcomes Quality Initiative (KDOQI) Clinical Practice Guidelines for Vascular Access have defied the access related care for patients with end-stage renal disease [1]. However, the standard of care across the world has fallen short of the KDOQI targets. One potential explanation for these shortcomings is the lack of compelling evidence in the literature to support the recommendations. ...
 

 Open Access DOI:10.23937/2572-4045.1510002

Stem Cell Transplantation (SCT) to Treat Severe Refractory Multiple Sclerosis (MS)

Athanasia Mouzaki

Article Type: Editorial | First Published: February 23, 2015

Multiple Sclerosis usually affects young adults aged between 20 to 40 years, although it can begin earlier or later in life. Women are affected almost twice as often as men. Symptoms include sensory disturbances in the limbs, optic nerve dysfunction, pyramidal tract dysfunction, bladder or bowel dysfunction, sexual dysfunction, ataxia and diplopia. ...
 

 Open Access DOI:10.23937/2572-4045.1510001

Auto Transplantation of Adipose and Adipose Derived Stem Cells

Jay M. Pensler

Article Type: Editorial | First Published: February 16, 2015

Auto transplantation of adipose cells and adipose derived stem cells is an increasingly popular procedure in Aesthetic Plastic Surgery. Utilizing liposuction to harvest an individual's fat the lipo-aspirate that was once harvested, is purified prior to auto transplantation. A number of techniques including low speed centrifugation, decanting, and/or blotting the various layers of the aspirated are utilized to increase the harvest of multi-potenti...

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