In the current paper, the first step for using Os-Pd/HfC nanocomposites as luminophore in producing Electrochemiluminescence (ECL) biosensor-namely increasing the sensitivity of biosensor through creating Os-Pd/HfC nanocomposites and using it instead of other nanocomposites for detecting and tracking of human gastroenterological cancer cells, tissues and tumors, is evaluated. Further, optimization of tri-n-propylamine (TPrA), 2-(dibutylamino) ethanol (DBAE) and benzyl peroxide (BPO) concentrations and Os-Pd/HfC nanocomposites as four main and effective materials in the intensity of luminescence for detecting and tracking of human gastroenterological cancer cells, tissues and tumors are considered so that the highest sensitivity obtains. In this regard, various concentrations of four materials were prepared and photon emission was investigated in the absence of human gastroenterological cancer cells, tissues and tumors.