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Journal of Sleep Disorders and Management




Polysomnography in a Large Population Based Study-the Study of Health in Pomerania Protocol

Beate Stubbe1#*, Thomas Penzel2#, Ingo Fietze2#, Anne Obst1#, Carmen Garcia2, Sandra Zimmermann2, Beate Diecker2, Martin Glos2, Carsten Oliver Schmidt3, Katharina Lau3, Michael Piontek3, Katrin Hegenscheid4, Johannes Dober4, Klaus Berger5,6, Andras Szentkiralyi5, Stephan B. Felix1, Christoph Schäper1, Sven Gläser1, Henry Völzke3,6,7 and Ralf Ewert1#


1Department of Internal Medicine B - Cardiology, Pneumology, Infectious Diseases, Intensive Care Medicine, University Hospital Greifswald, Greifswald, Germany
2University Hospital Charité Berlin, Sleep Center, Berlin, Germany
3Department of Community Medicine, SHIP/Clinical Epidemiology Research, University Greifswald, Greifswald, Germany
4Institute of Clinical Radiology and Neuroradiology, University Hospital Greifswald, Greifswald, Germany
5Institute of Epidemiology and Social Medicine, University of Muenster, Muenster, Germany
6German Centre for Diabetes Research, DZD, Partner Site Greifswald, Germany
7German Centre for Cardiovascular Research, DZHK, Site Greifswald, Germany
# These authors contributed equally to this work


*Corresponding author: Beate Stubbe (BS), MD, Department of Internal Medicine B, Cardiology, Pneumology, Infectious Diseases, Intensive Care Medicine, University Hospital Greifswald, F.-Sauerbruchstr, 17475 Greifswald, Germany, Tel: +49 3834 86 7242, Fax: +49 3834 86 7286, E-mail: beate.stubbe@uni-greifswald.de
J Sleep Disord Manag, JSDM-2-010, (Volume 2, Issue 1), Research Article
Received: March 22, 2016: Accepted: May 30, 2016: Published: June 02, 2016
Citation: Stubbe B, Penzel T, Fietze I, Obst A, Garcia C, et al. (2016) Polysomnography in a Large Population Based Study - the Study of Health in Pomerania Protocol. J Sleep Disord Manag 2:010.
Copyright: © 2016 Stubbe B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Abstract

Background:Our current knowledge about sleep disorders in the general population is limited by a lack of objective sleep data assessed in large-scale, well characterised European cohorts.

The Study of Health in Pomerania (SHIP) is a population-based study investigating prevalence and incidence of common risk factors, subclinical disorders and clinical diseases in adults. Polysomnography (PSG) was performed between 2008 and 2012 (SHIP-TREND).

Aim:The aim of the present study was to perform cardiorespiratory PSG in a population- based setting to gain more insight into subclinical and clinical sleep disorders and their complex associations with common risk factors, subclinical disorders and chronic diseases among adults. Here we describe the rationale, design, and implementation of PSG in a population-based study.

Methods:The Study of Health in Pomerania (SHIP) consists of two independent population-based prospective subcohorts. The core diagnostic program of the baseline SHIP-TREND included the assessment of risk behaviour, common chronic diseases, cardiac, vascular, pulmonary, and serum blood parameters, mental health, and cognitive functioning. Genotyping and whole-body MRI were also performed. In addition, all participants were offered a standard overnight laboratory-based polysomnography (PSG). Subjective sleep parameters and restless legs syndrome (RLS) were assessed with a set of standardised questionnaires. Sleep staging, respiratory events, and leg movements were evaluated according to the standards of the American Academy of Sleep Medicine (AASM).

Results:Complete data (sleep-questionnaires, PSG) have been obtained on 1249 participants (573 women), aged 20-70 years. Genetic data are available from 424 PSG participants.

Conclusion:The population-based design and the comprehensive data collection provide a unique opportunity to assess the prevalence and risk factors of sleep disorders, and to investigate the complex relationships between sleep parameters, genetic factors, and various health and disease aspects.


Keywords

Epidemiology, Polysomnography, Genetics, Restless-legs-syndrome


Background

Within the last 20 years technological progress of sleep-monitoring has improved diagnoses of sleep disorders [1]. The gold-standard for diagnosis and exclusion of sleep disorders is still the laboratory-based polysomnography (PSG). Besides improved technologies, standardised methods have greatly affected the quality of sleep recordings and were implemented into wide-accepted guidelines [1]. Sleep-related breathing disorders, such as obstructive sleep apnoea (OSA), are assumed to become further an increasing problem especially in the Western World as they are, besides diabetes, obesity and smoking, considered as relevant cardiovascular risk factors [2,3]. Large epidemiological studies have shown an association between OSAS and systemic hypertension in both general and sleep clinic populations [4-6]. However, many aspects of the relation between OSA and more specific cardiovascular risk factors are still unknown. Such factors are the extent of net blood pressure and other cardiopulmonary changes: the duration of OSA before inclusion, the percentage of hypertensive, overweight, and obese subjects, smoking status, alcohol use, and concomitant different medication therapy. In 1993, an estimated 2% of middle-aged women and 4% of middle-aged men (30 to 60 years) met the minimal diagnostic criteria for obstructive sleep apnea syndrome (OSAS) [7]. These were the occurrence of more than 5 apnea and hypopnea events per hour of sleep (apnea hypopnea index, AHI) plus symptoms of excessive sleepiness. If the occurrence of apneas and hypopneas without symptoms are taken into account, then 9% of women and 24% of men suffered from an AHI above 5 events per hour of sleep. Currently the prevalence of OSA with or without self reported symptoms is estimated to be up to 5% of the population of the Western world [8]. However, a vast majority of affected individuals still remains undiagnosed [9,10]. In the planning phase of the current study, a limiting factor of sleep research was the lack of large, well-characterised population samples i.e., in Europe who underwent PSG. Meanwhile epidemiological studies performed in Switzerland [11] and the US [12] contribute to prevalence aspects of sleep-disorders, especially sleep disordered breathing. A recent study on sleep disordered breathing and heart failure contributes to still needed more population based studies investigating the relationship between cardiovascular risk indicators and the severity of sleep disordered breathing [13]. Longitudinal analyses on associations between sleep disorders, morbidity and mortality within epidemiological studies as well as a better understanding of the meaning of subclinical disorders are still needed.

Associations of genetics and diverse aspects of sleep disorders have been described previously [14,15]. However, thorough knowledge on possible associations is still of interest.


Aims

The main objectives of PSG measurements in SHIP-TREND were:
1. to estimate the prevalence of sleep disorders,
2. to assess subjective and objective sleep quality,
3. to assess longitudinal associations between sleep disorders, mortality, and morbidity and
4. to assess and better understand possible associations of sleep disordered breathing with age, gender, genetic factors, risk behaviour, and co-morbidities in a German adult population-based cohort.

This paper outlines the study design and methods employed during the PSG assessments.


Cohort Characteristics

The Study of Health in Pomerania (SHIP) is a population-based, large-scale cohort project with two main objectives: (i) to assess prevalence and incidence of common risk factors, subclinical disorders and clinical diseases, and (ii) to investigate their complex associations [16]. The study region for SHIP is West Pomerania in the northeast of Germany. The project consists of two independent cohorts: a two-stage stratified cluster sample of 6265 adults 20-79 years has been drawn in 1996 (SHIP-0), a five-year follow-up has been performed 2002-2006 (SHIP-1), a ten-year follow-up has been performed 2008-2012 (SHIP-2). A separate stratified sample of 10,000 subjects was drawn from the central population registry in the federal state of Mecklenburg-Vorpommern, Germany (SHIP-TREND). Stratification variables were age, sex, and city/county of residence. Out of the net sample of 8826, after exclusion of deceased and relocated subjects 4,420 (2,275 women) participated (response 50.1%). The invitation procedure included three written invitations, phone calls, and home visits [16,17]. Baseline data collection for SHIP-TREND started in 2008 and examinations were finalised in 2012. In comparison to SHIP-0, further examination methods were introduced in SHIP-TREND, including PSG. All SHIP-TREND participants were offered to take part in the PSG subproject. Before PSG examinations, the subjects have undergone baseline examinations (anthropometric measurements, blood sampling etc.) and have filled in a sleep questionnaire. The comprehensive interview and core examination program, including whole body magnetic resonance tomography (MRI), cardiorespiratory, sonographic, neurological, dermatological, dental and ophthalmological examinations were done before PSG. Blood and urine samples were obtained according to standardized procedures. Serum, EDTA and citrate plasma, DNA and urine are stored at -80°C in a bio bank. In SHIP-TREND genotyping was performed in a subsample of 1000 subjects using Illumina Human Omni 2.5 Array (Illumina Inc., San Diego, CA, USA). For further details on the instruments of the SHIP and SHIP-TREND examination program see Völzke et al. [16].

A SHIP-TREND follow-up, called SHIP-TREND-1, has started in March 2016. A follow-up on polysomnography within this framework is planned.


PSG Examination

All SHIP-TREND participants were offered to undertake a full night attended PSG. Before PSG examinations, the subjects underwent baseline examinations (anthropometric measurements, blood sampling etc.) and completed a set of questionnaires regarding their sleep.

Data collection of sleep-characteristics comprised of the following components:
1. baseline interview,
2. sleep questionnaires,
3. attended PSG.

Within the complex framework of the SHIP-TREND protocol, the night in the sleep laboratory complied specific regularities. Either the participants were directly transferred from the main examination centre to the out-of-hospital located sleep laboratory or an appointment was made as defined by the participant. The median time between the main investigation and the PSG performance was nine days. Due to acclimatisation purposes PSG started at the earliest two to three hours after arrival of the participants in the sleep laboratory.


Interview

Trained and certified interviewers conducted the computer-assisted personal interviews, part of the baseline SHIP-TREND protocol (further details please see Völzke et al. [16]). Within the baseline interview five questions on sleep were included (Table 1).



Table 1: Sleep-questions of baseline SHIP-TREND interview. View Table 1


Standardized Sleep Questionnaires

The standardized sleep questionnaires were handed to PSG participants only. The following scales were part of the sleep questionnaires (Table 2):
1. Epworth Sleepiness Scale (ESS) [18],
2. Insomnia Severity Index (ISI) [19-21],
3. Pittsburgh Sleep Quality Index (PSQI) [22],
4. Restless Legs Syndrome-Diagnostic Index (RLS-DI) [23,24].



Table 2: Standardized sleep questionnaires. View Table 2


Laboratory-Based Attended PSG

Overnight attended PSG was conducted according to the standards of the American Academy of Sleep Medicine (AASM) [25] using ALICE 5 PSG devices (Philips Respironics, Eindhoven, The Netherlands). All sensors were carefully placed by trained and certified staff. Recordings included six channels of electroencephalogram (EEG, F4-M1, C4-M1, O2-M1, F3-M2, C3-M2, O1-M2, two channels of eletrooculogram (EOG), two channels of electromyogram (chin and tibialis muscles) [EMG], one electrocardiogram (ECG), inductive plethysmography detecting thoracic and abdominal movements, nasal pressure sensor, pulse oximetry, a microphone to detect snoring, and a body position sensor. Bio-calibration was performed before the light was switched-off (eye-movement, teeth grinding, breathing, snoring, and leg-movement). The variables documented or measured are listed in (Table 3). Up to four subjects were recorded in parallel per night. Participants were allowed to choose their own bedtime and to wake-up by themselves. However, due to reliability, a total in bed time of 8 hours was requested.



Table 3: Variables of PSG. View Table 3


PSG Analysis

All PSG recordings were digitally stored and transferred to the University Hospital Charité, Center of Sleep Medicine Berlin, Germany, where sleep stages of 30-second epochs and respiratory events were evaluated visually by certified sleep technicians according to AASM 2007 criteria. Analysis of these data provided information on central, mixed, and obstructive sleep apnoeas, about hypopnoeas, periodic breathing, hypoventilation periods, sleep efficiency, sleep states, arousals, heart rate variability, posture during sleep, snoring, leg movements and events and degree of oxygen desaturations. The scoring of sleep stages was performed visually according to the AASM criteria. Sleep stages were determined in terms of minutes and percentages. The analysis of pathological breathing events including differentiation between central, mixed and obstructive apnoeas and differentiation between hypopnoeas, periodic breathing and hypoventilation periods was done visually according to established criteria from the AASM [25]. Respiratory related arousals were also visually scored. The apnoea-hypopnoea index was calculated as the number of apnoes/hypopnoeas per hour of sleep estimated by the total sleep time. The severity of sleep apnoea was defined as follows based on the Apnoea-Hypopnoea index (AHI):
No sleep apnoea - AHI < 5 per hour sleep,
Mild sleep apnoea - AHI 5-15 per hour sleep,
Moderate sleep apnoea - AHI 15-30 per hour sleep,
Severe sleep apnoea - AHI ≥ 30 per hour sleep.

Periodic leg movements and related arousals were detected using an automatic algorithm according to the AASM criteria [25]. The periodic leg movement index (PLMI) was calculated as the number of leg movements appearing periodically and meeting the amplitude criteria according to the PLM-definition per hour of sleep.

The pulse transit time (PTT) was calculated for each heart beat based on the finger plethysmographic signal derived from pulse oximetry and the ECG signal. The system for this study has already an algorithm implemented to calculate PTT in a continuous way. The heart rate variability was calculated from ECG derived beat-to-beat intervals. Standard heart rate variability parameters as the low-frequency, the high-frequency component, and their ratio and mean heart rate values were calculated. In addition, new parameters derived using methods of statistical physics like long-range correlation, short-range correlation, and entropy were calculated, too.


MRI and PSG

Data provided by whole body magnetic resonance imaging (MRI) were used to investigate the influence of anatomical structures on the genesis of disorders such as obstructive sleep apnea. Volunteers that took part in both examinations (PSG and MRI) were selected for measurements focusing on the retropalatal oropharynx region. Five cephalometric parameters were chosen for further investigation and comparison with PSG. Data on both PSG and MRI are available from 701 adults (341 women).

On the midsagittal plane:
1. Minimal sagittal airway diameter- minimum distance between the posterior edge of the soft palate/uvula and the posterior pharyngeal wall.
On the transversal plane located at the mean distance between hard palate and tip of the uvula:
2. Lateral pharyngeal wall thickness- mean distance between the airway and the parapharyngeal fat pads.
3. Distance between the rami of the mandible-maximum distance between the inner sides of the most posterior parts of the rami of the mandible.
4. Distance between the fat pads-minimum distances between the parapharyngeal fat located lateral to the muscular pharyngeal wall.
5. Posterior airway wall thickness- distance between the airway and anterior edge of the spinal cord.

Intra- and inter-rater reliability was examined for chosen measurement methods and recorded data were archived in the SHIP database.

Further details on the MRI protocol are described by Hegenscheid et al. [26].


Quality Assurance

High-quality standards comprise an integral part of SHIP [1,27]. A standard operating procedure was established for PSG and MRI data measurement. The quality of sleep data was semi-annually discussed with independent scientists. Only certified personnel were allowed to conduct PSG. Initial certification of the PSG personnel was awarded to observers after a four weeks supervision by experienced personnel of the University Hospital Charité, Center of Sleep Medicine, Berlin, Germany. Furthermore, a 3-day education in the Center of Sleep Medicine Charité, Berlin was performed on the process of setting up and conducting PSG examination. During the study, certification procedures were repeated every 12 months, co-ordinated by the Sleep Centre Charité, Berlin. Inter- and Intra-Observer variability was determined for the PSG data and for the MRI image measurements. Investigators were instructed by the radiology staff of the University Hospital Greifswald also in charge of the SHIP MRI. A quality monitoring system was applied to examinations and readings to detect and describe any kind of methodological irregularities including missing data, extreme values, observer, and device variability. Based on this system a quarterly feedback was provided, and if necessary actions were taken. Challenges relating to the inter-scorer reliability between different sleep centres have been described previously [28]. Due to the single-centre assessment of PSG data applied within SHIP the reliability of the data is considered as excellent. Two special aspects of the data quality control have to be highlighted:

1. During data collection, locations of PSG assessments had to be changed twice within the city of Greifswald. All aspects of relocation (dates, rooms, facilities) were documented. Methodical implications of these relocations on data quality were not detected.

2. Inter- and intra-scorer reliability control revealed conspicuities in the visual evaluation of sleep stages. Therefore it was necessary to re-evaluate a couple of hundreds PSG data sets. The re-evaluation was performed by a scorer with low intra-scorer variability.

The very complex quality control protocol ensures that the data, released for analysis purposes, are of excellent quality.


Response and Attrition

Considerable effort was made to maximize subject response for SHIP-Trend and the PSG participation [29]. Individuals who gave consent to participate in the PSG examination were approached by three invitational letters and repeated telephone calls. All PSG participants were provided a breakfast, a short medical report of the PSG results and free transport to the examination centre and back home.

From the 4420 subjects (2275 women) participating in SHIP-TREND, 1630 individuals (754 women) volunteered for a PSG assessment (36.9%). Finally, 1249 (573 women) spent the night in the sleep laboratory (28.3%). Complete data on the parameters of breathing and EEG are available of 1209 individuals (559 women). Data on both PSG and MRI are available from 701 adults (341 women). Genetic data of the PSG participants are currently available from 424 individuals.


Strengths and Weaknesses

A major strength of the population-based SHIP-TREND is the comprehensiveness of information available on all aspects of socio-demography, health-status, risk factors and diseases in addition to polysomnographic sleep parameters. The SHIP-TREND is also notable for the high standards of quality assurance and the standardization of the applied methods. Much effort was put into personnel and structural resources. External facilities had to be rented only for the purpose of performing polysomnography within the framework of the study. Trained and certified students were deployed for the night watch. Separate work groups were responsible for data management and assessment.

A possible limitation is the potential selection bias due to the large proportion of individuals refusing to participate in the PSG assessment. In addition, the potential bias by selection to the PSG examination can be addressed by using the huge amount of data from the baseline examination. During data collection, locations of PSG investigations changed twice. However, all aspects of relocation (dates, rooms, facilities) were documented and methodical implications of these relocations on data quality were not detected. Finally, a single PSG night could be performed due to limited resources, whereas two consecutive nights would have been more optimal. We consider this a necessary trade-off in order to achieve a large number of PSG recordings in a population-based setting.


Acknowledgements

We gratefully acknowledge the contribution of field workers, study physicians, students and study nurses, computer scientists, medical documentarists and administration staff to data collection. Furthermore, we thank all study participants whose commitment and personal dedication have made this project possible.


Statement of Interest

The authors do not report any conflicts of interest.


Author's Role

All authors put substantial contribution to conception and design, analysis and interpretation of the data; on drafting the article and revising it critically for important intellectual content and final approval of the version to be published.


Funding

The work is part of the Community Medicine Research net (CMR) of the University of Greifswald, Germany, which is funded by the Federal Ministry of Education and Research (grants no. ZZ9603, 01ZZ0103, 01ZZ0403), Competence Network Asthma/ COPD (FKZ 01GI0881-0888), the Ministry of Cultural Affairs as well as the Social Ministry of the Federal State of Mecklenburg-West Pomerania. The CMR encompasses several research projects which are sharing data of the population-based Study of Health in Pomerania (SHIP; http://ship.community-medicine.de). This work was additionally funded by the Deutsche RLS e.V. (German Restless Legs Syndrome Society).


IRB Approval Statement

The study conformed to the principles of the Declaration of Helsinki as reflected by the approval by the IRB / Ethics Committee of the University of Greifswald ("Leben und Gesundheit in Vorpommern", III UV 73/01).


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