In the current paper, the Localized Surface Plasmon Resonance (LSPR) effect induced by Cadmium Oxide (CdO) nanoparticles is used to observe Raman spectrum of human cancer cells, tissues and tumors. The diagnosis and treatment of human cancer cells, tissues and tumors in sample is investigated through Nanomaterial Surface Energy Transfer (NSET) process from human cancer cells, tissues and tumors to the surface of nanoparticles, and Surface Enhanced Raman Scattering (SERS) process, as effective factors for Raman spectrum detection. For interaction of human cancer cells, tissues and tumors with Cadmium Oxide (CdO) nanoparticles, colloidal state and Self-Assembled Monolayer (SAM) methods were used. Both methods have shown good agreement with each other in detecting the Raman spectrum. It should be noted that these methods and techniques can be applied on different types of human's cancer cells, tissues and tumors, respectively.