Can BU, Özbay G, Altundal H, Durmazpinar PM, Kitiki B, et al. (2018) Evaluation of Synergetic Antibacterial Effect of Gaseous Ozone and Ozonated Water against Enterococcus Faecalis in Human Root Canals. Int J Oral Dent Health 4:076.


© 2018 Can BU, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

RESEARCH ARTICLE | OPEN ACCESSDOI: 10.23937/2469-5734/1510076

Evaluation of Synergetic Antibacterial Effect of Gaseous Ozone and Ozonated Water against Enterococcus Faecalis in Human Root Canals

Banu Uygun Can1, Gizem Özbay2, Hatice Altundal2, Parla Meva Durmazpinar3*, Burak Kitiki2, Sertaç Peker2, Tanju Kadir1 and BetÜl Kargul4

1Department of Microbiology, Marmara University, Turkey

2Private Practice, Istanbul, Turkey

3Department of Endodontics, Izmir Demokrasi University, Turkey

4Department of Pediatric Dentistry, Marmara University, Turkey



Elimination of the microorganisms in root canals is one of the most important stages for a successful endodontic treatment. Several techniques and agents have been tested to find an alternative option to sodium hypochloride for effective root canal disinfection. The aim of this in vitro study was to evaluate the antimicrobial efficacy of gaseous ozone and ozonated water in order to establish their potential as endodontic root canal disinfectant.


Eighty teeth with single canal were prepared and inoculated with Enterococcus faecalis suspension and incubated for a week. The contaminated root canals were exposed to irrigating solutions including gaseous ozone (44 µg ml-1) with ozonated water (5 µg ml-1), 5.25% sodium hypochlorite, 2% chlorhexidine for 3 min. Samples from the root canals were collected by paper points, before and after irrigation. The bacterial count was analyzed by serial decimal dilutions and the percentage of surviving microorganisms was statistically assessed.


There was a significant reduction in the bacterial count after all treatments (P < 0.001) while having greater antimicrobial reduction was observed on samples of NaOCl irrigation. There was no significant difference between gaseous ozone and gaseous ozone + ozonated water groups.


Under limitations of this study, it could be concluded that the gaseous ozone, as an irrigant agent, significantly reduced the number of E. faecalis in root canals but was not able to eliminate it. However, ozonated water was insufficient to increase the antibacterial effectiveness of gaseous ozone.