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International Journal of
Tropical Diseases ISSN: 2643-461X

Citation

Moss DM, Whitney MT, Chard AN, Trinies V, Doumbia S, et al. (2018) Serological Evidence of Dengue and Chikungunya Exposures in Malian Children by Multiplex Bead Assay. Int J Trop Dis 1:007. doi.org/10.23937/ijtd-2017/1710007

Copyright

© 2018 Moss DM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

RESEARCH ARTICLE | OPEN ACCESSDOI: 10.23937/ijtd-2017/1710007

Serological Evidence of Dengue and Chikungunya Exposures in Malian Children by Multiplex Bead Assay

Delynn M Moss1*, Matthew T Whitney2, Anna N Chard3, Victoria Trinies3, Seydou Doumbia4, Christin H Goodman2, Stevan Bullard5, Ryan E Wiegand6, Matthew C Freeman3, Patrick J Lammie6and Gwong-Jen Chang2

1Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Georgia, USA

2Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Georgia, USA

3Department of Environmental Health, Rollins School of Public Health, Emory University, Georgia, USA

4Malaria Research and Training Center, Faculty of Medicine and Odonto stomatology, University of Sciences, Techniques and Technologies of Bamako (USTTB), Mali

5Division of Toxicology and Human Health Sciences, Geospatial Research, Analysis and Services Program, Centers for Disease Control and Prevention, Georgia, USA

6Division of Parasitic Diseases and Malaria, Centers for Global Health, Centers for Disease Control and Prevention, Georgia, USA

Abstract

We opportunistically included a chikungunya envelope 1 recombinant virus antigen (CHIKV E1), a dengue 2 virus-like protein (DENV-2 VLP), and a DENV-3 VLP in a multiplex bead assay (MBA) for serological assessment involving improvements in water, sanitation, and hygiene for students attending 42 elementary schools in southern Mali in Western Africa. From students attending grades 1-6,805 dried blood spots (DBS) were collected. Immunoglobulin (Ig) G was eluted from the DBS and was probed for responses to the antigens. For all students, positive IgG responses to CHIKV E1, DENV-2 VLP, and DENV-3 VLP were 6.2%, 24.3%, and 14.8%, respectively. However, in some schools the positivity rate was as high as 21.1% for CHIKV E1 and 66.7% for both DENV-2 and DENV-3 VLP. In one set of schools, cluster analysis from SaTScan software showed a relative risk of 3.1 and 5.1 for DENV-2 and DENV-3 VLP, respectively, compared with schools outside the cluster. The MBA serology platform is very flexible in that additional antigens other than the intended targets can be included with very little cost and can provide additional effective opportunities for evaluation of public health activities.