The overuse of antibiotics due to poor health and suffering is a threat to global health. In Vietnam, overuse is a widespread problem because of the high availability of antibiotics and lack of knowledge about their use, and nurses have a significant role in informing and helping patients experience higher levels of health and well-being. The aim of this study was to describe Vietnamese nursing students' experience with antibiotics in healthcare. A qualitative content analysis with an inductive approach was used based on six semistructured interviews with nursing students at Hanoi Medical University in Vietnam. The results formed one category, "Uncertainty regarding antibiotic use", and three subcategories: "To obtain limited knowledge", "To contribute to improved knowledge", and "To manage self-medication without the support of a health professional". Limited knowledge influences health professionals as well as patients and causes poor health, which contributes to the nonprofessional use of antibiotics. In conclusion, increased learning regarding antibiotics (e.g., evidence-based knowledge) as well as pedagogic skills and a sense of coherence (SOC) can be used to reduce the overuse of antibiotics, and interprofessional health education can facilitate patient education and professional teamwork.