Target genes for PCR, reagents' volume used for each round and cycling conditions.

Table 1: Target genes for PCR, reagents' volume used for each round and cycling conditions.

Round	Target Gene	Reagents' volume	Cycling condition
1	ITS1 and ITS2	6.25 μl Dream Taq master mix, 0.25 μl ITS1 and ITS2 primers, 3.25 μl of nuclease free water and 2.5 μl template DNA	35 cycles with cycling condition of 95 ℃ for 7 minutes then 94 ℃ for 1 min, 55 ℃ for 1 min, 72 ℃ for 2 minutes
2	ITS3 and ITS4	6.25 μl Dream Taq master mix, 0.25 μl ITS3 and ITS4 primers, 5.25 μl of nuclease free water and 0.5 μl