Table 1: Cycling conditions for nested ITS-PCR and expected band sizes for each trypanosoma species.
| Trypanosoma species | Band size (bp) | Cycling conditions |
|---|---|---|
| T. congolense (Forest) T. congolense (Kilifi) T. congolense (Savannah) T. brucei s.i. T. theileri T. simiae (Tsavo) T. simiae T. vivax |
1513 bp 1422 bp 1413 bp 1207-1224 bp 988 bp 954 bp 850 bp 611 bp |
Two rounds, 35 cycles, each. One cycle: 95 °C for 7 mins 94 °C for 1 mins 55 °C for 1 mins 72 °C for 2 mins |