Table 3: Cluster of hot spot residues on FANCD2 interface of interaction.

Chain Residue KFC2-A* KFC2-B** Partner
a ARG 591 0.25 0.30 RAD51a
a HIS 594# 0.96 0.24 RAD51a
a GLN 597 0.56 0.02 RAD51a
a ARG 648 0.82 0.10 RAD51a
a ILE 849# 0.32 0.05 RAD51a
a ILE 855# 0.64 0.16 RAD51a
a ARG 879# 1.12 0.34 RAD51a
a ARG 886# 0.74 0.32 RAD51a
a LEU 889 1.74 0.31 RAD51a

*Hot spot model based on shape specificity features; **Hot spot model based on biochemical features such as intermolecular hydrogen bonds; #Polymorphic residues that may greatly affect the free energy binding of FANCD2 to RAD51a.