Table
1: SNP coding region, primer sequences and final
concentration for PCR amplification (according to Alvarez-Iglesias et al [19]).
Nucleotide Position |
Primer Sequences (5’®3’) |
Amplicon length (bp) |
Final Concentration (µM) |
Annealing Temperature (°C) |
1119 |
GCTAAGACCCAAACTGGGATT GTTCTGGCGAGCAGTTTTGT |
84 |
0.5 |
56 |
2092 |
TGCCCACAGAACCCTCTAAA TGTCCAAAGAGCTGTTCCTCT |
69 |
0.5 |
60 |
4491 |
ATTAATCCCCTGGCCCAAC GATGAGTGTGCCTGCAAAGA |
56 |
0.2 |
57 |
5417 |
CCTCAATCACACTACTCCCCATA GGGGTGGGTTTTGTATGTTC |
72 |
0.5 |
56 |
8281-8289del |
TAGGGCCCGTATTTACCCTAT AAGAGGTGTTGGTTCTCTTAATCTTT |
109 |
0.5 |
55 |
8414 |
TACTACCGTATGGCCCACCA GGGAGGTAGGTGGTAGTTTGTG |
96 |
0.2 |
57 |
9824 |
TTTTGTAGCCACAGGCTTCC GAGGAAAGTTGAGCCAATAATGA |
53 |
0.4 |
56 |
13759 |
GGAAGCCTATTCGCAGGATT GTTGTTTGGAAGGGGGATG |
64 |
0.4 |
57 |
12705 |
CCCAAACATTAATCAGTTCTTCAA TCTCAGCCGATGAACAGTTG |
102 |
0.4 |
56 |