Table 2:  Amount of Viral Protein.  Virus proteins detected by mass spectrometry were quantified.  The amount of viral protein (femto-moles of HA, NA, NP or M1) detected per nanogram of purified virus loaded is shown.  The amount of hemagglutinin and neuraminidase protein is expressed as a ratio with either the NP or M1 proteins.  ND: where viral protein was not detected the ratio was not determined.

Virus

 

fmol/ng

HA:NP

NA:NP

HA:M1

H1N1 A/Bolivia/559/2013

Egg

4.51

0.6

0.35

0.18

Cell

1.29

ND

ND

5.8

H3N2 A/New York/39/2012

Egg

7.07

0.38

0.14

0.24

Cell

0.12

ND

ND

ND

B/Massachusetts/02/2012

Egg

5.94

7.7

0.70

1.9

Cell

22.5

3.6

0.28

2.7