Heath Risk Assessment of Heavy Metals in Shrimp Acetes sp . and Cincalok Collected in 2007 from Pantai Klebang, Peninsular Malaysia

Samples of shrimp ( Acetes Sp. ), were collected from Pantai Klebang (Malacca, Peninsular Malaysia) in 2007. The mean metal concentrations (µg/g dry weight) in the total tissue of the shrimps were 1.47 for Cd, 7.10 for Cu, 41.5 for Fe, 3.70 for Ni, 4.49 for Pb and 27.1 for Zn. The metal levels (µg/g dry weight) in the shrimps are lower than Cd (1.47), Cu (8.55), Ni (4.19), and Pb (4.86). However, the levels of Fe (21.2) and Zn (11.0) in the cincalok were lower than those in the collected shrimps. For the human health risk assessment, all values of target hazard quotient for the six metals in the adults of Malaysian population were found below 1.00. This showed that there was no non-carcinogenic risk of the six metals via the consumption of Acetes Sp. from the present study. However, regular monitoring of heavy metals in this popular small shrimps is still needed.


Introduction
In Malaysia, the geragau Acetes sp. (Family: Sergestidae) were focused in the present study because these wild small shrimps are heavily fished in terms of total tonnage. They are natural benthic omnivores of the coastal areas of Peninsular Malaysia and hold signifi-reported as Acetes sp.
Determination of total tissue dry weight (dw) were conducted by drying individual total tissue at 60 °C, for 72 hours until constant dw was achieved [4]. The cincalok and the fresh samples of Acetes sp. were placed onto aluminum foils dried at 105 °C, for 72 hours until constant dw was achieved [4].
The dissected dried tissues (about 0.5 g each) were weighed and digested in concentrated nitric acid (AnalaR grade, BDH 69%). Digestion were carried out by heating the nitric acid treated samples at 40 °C for 1 hour and then raised to 140 °C for at least 3 hours to achieve full digestion. After the digestion process was completed, the samples were topped up with DDW to 40 ml and before filtering them with Whatman No.1 (filter speed: medium) filter papers into acid washed plastic containers. The digested samples were then sent for Cd, Cu, Fe, Ni, Pb and Zn determination by using the atomic absorption spectrophotometer (AAS) Perkin-Elmer Model 800.
All glassware and equipment used for this study were acid washed and procedural blanks were analyzed once for every five samples during analysis to ensure consistency in data collection. Standard solutions for all six metals were prepared and analyzed occasionally during the analysis for heavy metal recoveries. The heavy metal recoveries were being satisfactory with recoveries between 90-110%. The quality of the analytical procedures used for the shrimp samples were verified by using CRM for Dogfish Liver (DOLT-3, National Research Council Canada). The recoveries of the six metals for the CRMs were being satisfactory between 80-120%.

Human Health Risk Assessment
The estimated daily intake is to calculate how much of shrimps that be taken by an adult for one day. First, the dry weight (dw) basis was converted to wet weight (ww) by using the conversion factor (0.30) as follows: Wet weight (WW) = metal concentration (MC) × conversion factor The mean concentrations of the samples are needed for calculation of estimated daily intake of shrimps. The estimated daily intake (EDI) (µg/kg/day) of shrimps that contain levels of Cd, Cu, Fe, Ni, and Zn were measured by using a formula: MC represents the metal concentration (µg/g wet weight) in the shrimps. The body weight (BW; kg) for adults is 62 kg and consumption rate (CR; g/person/ day) for cephalopod is 90 g, following the report for Malaysian population [5].
The human health risk assessment of the heavy metal in the guava fruits was determined by the calculation of Target Hazard Quotient (THQ). If the THQ value is higher than 1.0, this means that the daily consumption of shrimps would likely result in negative health effects during a lifetime in a human population [6]. The formula of THQ calculation was described as follow:

Results and Discussion
The mean concentrations of Cd, Cu, Fe, Ni, Pb and Zn in Acetes sp. and cincalok from Pantai Klebang are presented in Table 1. The mean metal concentrations (µg/g dry weight) in the total tissue of the shrimps were 1.47 for Cd, 7.10 for Cu, 41.5 for Fe, 3.70 for Ni, 4.49 for Pb and 27.1 for Zn. The metal levels (µg/g dry weight) in the shrimps are lower than Cd (1.47), Cu (8.55), Ni  [8].
The values of EDI and THQ of Cd, Cu, Fe, Ni, Pb and Zn of Acetes sp., from the present study and cited data from the literature, are shown in Table 2. All values of THQ for the six metals in the adults of Malaysian population were found below 1.00. This shows that there was no non-carcinogenic risk of the six metals via the consumption of Acetes sp. from the present study.
The results showed that the cincalok and geragau. accumulated different levels of metals. Higher accumulation of Cd, Cu, Ni and Pb could be seen in cincalok rather that the geragau, which the raw Acetes as a major ingredient shrimp for the making of cincalok. This could be due to the preservatives added and contamination during the making of the cincalok.
Rahouma, et al. [2] reported that the concentrations of heavy metals of Acetes shrimp collected from Malacca, Terengganu and Johor in 2011, were safe and MPLs for the human consumption and public health. Rahouma, et al. [1] reported that the metal levels in Acetes indicus sampled from Kedah and Malacca were safe for human consumption since they were within the MPL.  1 Rahouma, et al. [1] for Malacca and Kedah; 2 Anandkumar, et al. [3]; 3 Rahouma, et al. [2] for Malacca and Terengganu.