Table 2: Semi-quantitative IHC score of stem cell markers, osteogenic markers, and endothelial markers (Standard abbreviation in brackets; p = p-value).
Static conditions | Dynamic conditions | |||||||||
Culturing conditions | Day 4 | Day 10 | Day 14 | Day 10 | Day 14 | |||||
Stem cell markers (p = 1.9 × 10-5) | ||||||||||
CD13 | 3.5 | (± 1.2) | 4 | (± 0) | 3.5 | (± 1.2) | 4 | (± 0) | 4 | (± 0) |
CD90 | 0.7 | (± 0.5) | 0.8 | (± 0.8) | 0.8 | (± 0.5) | 0.7 | (± 0.5) | 1 | (± 0.6) |
CD44 | 1 | (± 1.6) | 2.2 | (± 1.2) | 2.3 | (± 1.9) | 3.2 | (± 1.2) | 3 | (± 0) |
Osteogenic markers (*) (p = 0.013) | ||||||||||
Osteopontin | 3.8 | (± 0.4) | 3.8 | (± 0.4) | 2.2 | (± 0.5) | 3.8 | (± 0.4) | 4 | (± 0) |
Osteonectin | 4 | (± 0) | 4 | (± 0) | 4 | (± 0) | 4 | (± 0) | 4 | (± 0) |
Endothelial markers (**) (p = 0.029) | ||||||||||
CD34 | 0.5 | (± 0.5) | 0.5 | (± 0.8) | 0.4 | (± 0.7) | 0.3 | (± 0.5) | 0.9 | (± 1.4) |
vWF | 0.2 | (± 0.4) | 0 | (± 0) | 0.5 | (± 0.6) | 0.2 | (± 0.4) | 0 | (± 0) |
*Collagen I was not analyzed due to the collagen origin of the scaffolds; Osteocalcin was not detectable; **CD31 was not detectable. |