Table 1: Primers used for RT-qPCR.
Gene | Primer sequence |
Housekeeping genes | |
GAPDH-F | 5'-CTCAGACACCATGGGGAAGG-3' |
GAPDH-R | 5'-TCGCTCCTGGAAGATGGTGA-3' |
RNA18S5-F | 5'-AGAAACGGCTACCACATCCA-3' |
RNA18S5-R | 5'-CCCTCCAATGGATCCTCGTT-3' |
Stem cell marker | |
CD44-F | 5'-CCCATTCGACAACAGGGACA-3' |
CD44-R | 5'-TGGGGTGTGAGATTGGGTTG-3' |
ANPEP-F | 5'-TCAACTACACCCTCAGCCAG-3' |
ANPEP-R | 5'-ATTGCCCTCCATGTACTCGC-3' |
THY1-F | 5'-CAGCATCGCTCTCCTGCTAA-3' |
THY1-R | 5'-ACTGGATGGGTGAACTGCTG-3' |
Osteogenic marker | |
BGLAP-F | 5'-ACACTCCTCGCCCTATTGGC-3' |
BGLAP-R | 5'-GATGTGGTCAGCCAACTCGT-3' |
SPP1-F | 5'-CATCACCTGTGCCATACCAGTT-3' |
SPP1-R | 5'-TTGGAAGGGTCTGTGGGGCTA-3' |
COL1A1-F | 5'-CCACCAATCACCTGCGTACA-3' |
COL1A1-R | 5'-GGCAGTTCTTGGTCTCGTCA-3' |
Endothelial marker | |
CD34-F | 5'-AAGCCGAGTAGTGTCTTCCAC-3' |
CD34-R | 5'-GGGGTAGCAGTACCGTTGTT-3' |
PECAM1-F | 5'-TCCCCTAAGAATTGCTGCCA-3' |
PECAM1-R | 5'-TCTTCCCAACACGCCAATGA-3' |
VWF-F | 5'-ATGCCCCTGGAGAAACAGTG-3' |
VWF-R | 5'-CCGAAAGGTCCCAGGGTTAC-3' |