Table 1: Primer sequences for real-time, RT-PCR.

Gene		Primer sequence (5' to 3')	Annealing temperature (°C)
H1R	forward reverse	GCCGTCGTCATGCCTATGA GAGAGGACGGCCCAGTGA	62°C
occludin	forward reverse	ATCTGACTATGTGGAAAGAAAA ACCGCTTGTCATTCACTTTG	58°C
ZO-1	forward reverse	CGGTCCTCTGAGCCTGTAAGGGATCTACATGCGACGACAA	58°C
MMP-1	forward reverse	CCTCGCTGGGAGCAAACA TTGGCAAATCTGGCGTGTAAT	59.3°C
VEGF	forward reverse	TCCTCACACCATTGAAACC TGGAGGAAGGTCAACCACT	59.6°C
IL-6	forward reverse	AAGCCAGAGCTGTGCACATGAGTA TGTCCTGCAGCCACTGGTC	60°C
GAPDH	forward reverse	GCACCGTCAAGGCTGAGAAC TGGTGAAGACGCCAGTGGA	60°C

H1R: Histamine Receptor 1; GAPDH: Glyceraldehyde-3-Phosphate Dehydrogenase; IL: Interleukin; MMP-1: Matrix Metalloprotease; VEGF: Vascular Endothelial Growth Factor; ZO-1: Zona Occludens Protein 1.