Gene | Primer sequence (5' to 3') | Annealing temperature (°C) | |
H1R | forward reverse |
GCCGTCGTCATGCCTATGA GAGAGGACGGCCCAGTGA |
62°C |
occludin | forward reverse |
ATCTGACTATGTGGAAAGAAAA ACCGCTTGTCATTCACTTTG |
58°C |
ZO-1 | forward reverse |
CGGTCCTCTGAGCCTGTAAGGGATCTACATGCGACGACAA | 58°C |
MMP-1 | forward reverse |
CCTCGCTGGGAGCAAACA TTGGCAAATCTGGCGTGTAAT |
59.3°C |
VEGF | forward reverse |
TCCTCACACCATTGAAACC TGGAGGAAGGTCAACCACT |
59.6°C |
IL-6 | forward reverse |
AAGCCAGAGCTGTGCACATGAGTA TGTCCTGCAGCCACTGGTC |
60°C |
GAPDH | forward reverse |
GCACCGTCAAGGCTGAGAAC TGGTGAAGACGCCAGTGGA |
60°C |