Table 1: Primer sequences for real-time, RT-PCR.
Gene Primer sequence (5' to 3') Annealing temperature (°C)
H1R forward
reverse
GCCGTCGTCATGCCTATGA
GAGAGGACGGCCCAGTGA
62°C
occludin forward
reverse
ATCTGACTATGTGGAAAGAAAA
ACCGCTTGTCATTCACTTTG
58°C
ZO-1 forward
reverse
CGGTCCTCTGAGCCTGTAAGGGATCTACATGCGACGACAA 58°C
MMP-1 forward
reverse
CCTCGCTGGGAGCAAACA
TTGGCAAATCTGGCGTGTAAT
59.3°C
VEGF forward
reverse
TCCTCACACCATTGAAACC
TGGAGGAAGGTCAACCACT
59.6°C
IL-6 forward
reverse
AAGCCAGAGCTGTGCACATGAGTA
TGTCCTGCAGCCACTGGTC
60°C
GAPDH forward
reverse
GCACCGTCAAGGCTGAGAAC
TGGTGAAGACGCCAGTGGA
60°C


H1R: Histamine Receptor 1; GAPDH: Glyceraldehyde-3-Phosphate Dehydrogenase; IL: Interleukin; MMP-1: Matrix Metalloprotease; VEGF: Vascular Endothelial Growth Factor; ZO-1: Zona Occludens Protein 1.