Table 1:
Antibody source, clone, and dilution and antigen retrieval method for immunohistochemical analysis.
Antibody |
Source |
Clone |
Dilution |
Antigen retrieval method |
p63 |
Santa Cruz |
4A4 |
1:1000 |
Citrate buffer, 100°C, 1 min |
Cytokeratin 5/6 |
Dako |
D5/16B4 |
1:50 |
Citrate buffer, 100°C, 20 min |
c-kit |
Dako |
Polyclonal |
1:100 |
Citrate buffer, 100°C, 15 min |
EGFR |
Invitrogen |
31G7 |
1:50 |
Proteinase K, 10 min |
GATA-3 |
Santa Cruz |
HG3-31 |
1:100 |
Tris-EDTA, 100°C, 15 min |
Synaptophysin |
Novocastra |
27G12 |
1:600 |
Citrate buffer, 100°C, 35 min |
Chromogranin A |
Chemicon |
LK2H10 |
1:4000 |
Citrate buffer, 100°C, 20 min |
Ki-67 |
Dako |
MIB-1 |
1:100 |
Tris-EDTA, 100°C, 20 min |
Estrogen receptor |
Novocastra |
6F11 |
1:35 |
Citrate buffer, 100°C, 30 min |
Progesterone receptor |
Dako |
PgR1294 |
1:200 |
Citrate buffer, 100°C, 20 min |
Her2 |
Labvision |
E2-400 |
1:300 |
Citrate buffer, 100°C, 9 min |