Table 1: Antibody source, clone, and dilution and antigen retrieval method for immunohistochemical analysis.

Antibody

Source

Clone

Dilution

Antigen retrieval method

p63

Santa Cruz

4A4

1:1000

Citrate buffer, 100C, 1 min

Cytokeratin 5/6

Dako

D5/16B4

1:50

Citrate buffer, 100C, 20 min

c-kit

Dako

Polyclonal

1:100

Citrate buffer, 100C, 15 min

EGFR

Invitrogen

31G7

1:50

Proteinase K, 10 min

GATA-3

Santa Cruz

HG3-31

1:100

Tris-EDTA, 100C, 15 min

Synaptophysin

Novocastra

27G12

1:600

Citrate buffer, 100C, 35 min

Chromogranin A

Chemicon

LK2H10

1:4000

Citrate buffer, 100C, 20 min

Ki-67

Dako

MIB-1

1:100

Tris-EDTA, 100C, 20 min

Estrogen receptor

Novocastra

6F11

1:35

Citrate buffer, 100C, 30 min

Progesterone receptor

Dako

PgR1294

1:200

Citrate buffer, 100C, 20 min

Her2

Labvision

E2-400

1:300

Citrate buffer, 100C, 9 min