Table 2: Biomarker data.
|
AE1/3 |
|
BerEP4 |
|
|
|||||
|
Sample
ID |
Normalised & transformed RPPA fluorescence |
%CV |
Normalised pixel count |
Mean
Pixel intensity |
|
Normalised & transformed RPPA fluorescence |
%CV |
Normalised pixel count |
Mean
Pixel intensity |
|
1 |
24.08063 |
2.19 |
666.411 |
149.865 |
|
98.37199 |
3.01 |
545.300 |
146.440 |
|
2 |
29.99488 |
2.34 |
527.311 |
164.432 |
|
130.8607 |
10.4 |
520.600 |
159.335 |
|
3 |
32.2181 |
11.17 |
316.226 |
178.507 |
|
136.1842 |
9 |
323.271 |
170.675 |
|
4 |
26.29081 |
3.05 |
456.912 |
169.327 |
|
120.9681 |
8.46 |
431.443 |
150.950 |
|
5 |
25.54104 |
13.44 |
297.248 |
184.110 |
|
107.4351 |
1.93 |
269.962 |
175.588 |
|
6 |
21.45467 |
0.7 |
193.192 |
198.573 |
|
91.46121 |
9.05 |
138.992 |
209.361 |
|
7 |
39.00818 |
7.86 |
519.455 |
159.385 |
|
169.1949 |
3.29 |
537.467 |
154.825 |
|
8 |
25.02173 |
12.7 |
169.643 |
202.428 |
|
100.4933 |
10.22 |
125.871 |
200.004 |
|
9 |
16.23427 |
6.23 |
213.207 |
198.312 |
|
69.79275 |
11.08 |
222.248 |
181.677 |
|
10 |
17.44557 |
6.73 |
421.015 |
180.545 |
|
71.54607 |
11.32 |
383.315 |
171.640 |
|
11 |
42.7618 |
1.49 |
766.985 |
153.429 |
|
167.1932 |
0.84 |
592.467 |
144.325 |
|
12 |
22.8176 |
2.36 |
506.670 |
158.613 |
|
90.34716 |
10.54 |
413.615 |
175.365 |
|
13 |
23.50721 |
4.11 |
332.619 |
168.408 |
|
100.3395 |
9.67 |
334.126 |
164.713 |
|
14 |
38.77023 |
11.26 |
491.610 |
148.419 |
|
143.2663 |
10.58 |
500.110 |
137.885 |
|
15 |
17.10665 |
1.55 |
266.242 |
182.767 |
|
71.44505 |
1.56 |
331.304 |
219.324 |
|
16 |
15.30348 |
7.06 |
384.530 |
188.633 |
|
54.05024 |
8.73 |
305.249 |
172.089 |
|
17 |
28.46959 |
4.93 |
711.911 |
134.989 |
|
107.4079 |
1.56 |
716.140 |
111.011 |
|
18 |
17.30357 |
7.85 |
397.970 |
167.113 |
|
71.59679 |
4.75 |
358.735 |
162.565 |
|
19 |
21.23668 |
4.9 |
592.412 |
157.987 |
|
97.10252 |
5.62 |
520.086 |
156.988 |
This shows the expression
of AE1/3 and BerEP4 by RPPA and by IHC. The RPPA data have been normalised for protein loading and have undergone square
root transformation to allow generate parametric data. %CV is the coefficient
of variation calculated for replicates for each sample and derived using raw
fluorescence data. For the IHC data, normalised pixel
count refers to the number of positive pixels normalised
to the total number of pixels of the tissue section. Mean pixel intensity is
derived from only the pixels which were positive in the tissue section.