Table 1: The effect of conjugate dilution on ELISA absorbance for positive melioidosis and normal control sera at 60 minutes after adding substrate and the antigen concentration was 0.050 mg/mL

Conjugate
1:4000
1:2000
Dilutions Positive melioidosis Normal control *Percentage (%) Positive melioidosis Normal control *Percentage (%)
1:80 0.987 0.629 35.8 1.09 0.812 27.8
1:160 0.89 0.497 39.3 0.976 0.685 29.1
1:320 0.884 0.379 50.5 0.939 0.574 36.5
1:640 0.863 0.29 57.3 0.92 0.487 43.3
1:1280 0.85 0.236 61.4 0.91 0.323 58.7
1:2560 0.843 0.211 63.2 0.784 0.24 54.4
1:5120 0.739 0.171 56.8 0.619 0.197 42.2
1:10240 0.56 0.141 41.9 0.442 0.108 33.4
1:20480 0.428 0.13 29.8 0.265 0.102 16.3
1:40960 0.302 0.112 19.0 0.229 0.095 13.4
1:81920 0.25 0.112 13.8 0.179 0.094 8.5
*Percentage of absorbance difference between positive melioidosis and normal control