Table 1: Results of Line probe assay proficiency testing pilot

Proficiency testing Attempt Total tests conducted Negative control Invalid results (number invalid/total number, %) Internal concordance (same patient, same laboratory)* External concordance (same specimen, different laboratory)* Comments Outcome of proficiency testing attempt
Benchmark Clean ≤10% ≥ 95% ≥ 95%
Lab A 1st 48 Contamination 14/ 48 (29%) - - Terminated due to contamination Failed: Repeat
2nd 22 Clean 15/22 (70%) - - Terminated due to high invalidity rate as benchmark could not be achieved Failed:Repeat
3rd 100 Clean 4/100 (4%) 48/48 (100%)* 19/19 (100%)* * One sample excluded due to invalid result Proficiency achieved
Lab B 1st 100 Clean 15/100(15%) 36/37 (97%)* 17/17 (100%)* 17/17 (100%)* Failed: Repeat
2nd 100 Clean 6/100 (6%) 46/46 (100%)* 20/20 (100%) Proficiency achieved
Lab C 1st 100 Clean 7/100 (7%) 46/46 (100%)* 19/20 (95%) Proficiency achieved
Lab D 1st 96 Contamination 24/96 (25%) Terminated due to contamination Failed:Repeat
Lab E 1st 100 Clean 4/100 (4%) 48/48(100%)* 20/20 (100%) Proficiency achieved
Lab F 1st 50 Clean 11/50(22%) Terminated due to high invalidity rate as benchmark could not be achieved Failed:Repeat
2nd 100 Clean 2/100(2%) 47/48 (97.9%)* 19/20 (95%) Proficiency achieved