List of qRT-PCR and sequencing primers of target and reference genes.

Table 1: List of qRT-PCR and sequencing primers of target and reference genes.

GENE	Direction	Sequence (5'-3')	Tm	Amplicon size (bp)
		Real-time qRT-PCR primers
		House-keeping genes
18SrRNA	FW	GGCGCCCCCTCGATGCTCTTAG	62	89
	RV	GCTCGGGCCTGCTTTGAACACTCT	61
28SrRNA	FW	TGTCGGCTCTTCCTATCATTGT	53	81
	RV	ACCCAGCTCACGTTCCCTATTA	55
ACTB	FW	GTTGTCGACGACGAGCG	52	93
	RV	GCACAGAGCCTCGCCTT	52
GAPDH	FW	TTGAGGTCAATGAAGGGGTC	52	117
	RV	GAAGGTGAAGGTCGGAGTCA	54
		Target genes
DMT1-NIRE	FW	TCCCCATGATCTCCAGAAAC	52	115
	RV	GGTTGCGGAGCTGGTAAG	53
DMT1-IRE	FW	TGCACCATGAGGAAGAAGCA	52	134
	RV	ACACTGTCATCTGACATCTTCTGT	57
TFR2	FW	AATGTCCTGAGTCAGAGCGG	56	141
	RV	GGCAGACTCTACTGGAGCGA	56
FPN	FW	ATGGGGGCTAAGATGTTGGT	52	108
	RV	CCAAAGGGATTGGATTGTTG	50
FTH	FW	TGGTTCTGCAGCTTCATCAG	52	148
	RV	TGGAGCTCTACGCCTCCTAC	56	56
FTL	FW	GCCTGGTTCAGCTTTTTCTC	52	145
	RV	GCTACGAGCGTCTCCTGAAG	56
IRP1	FW	CAATGGCTCAGCAAGGTGT	51	108
	RV	CTGCTTGGGTCAGGTTCG	53
IRP2	FW	AGCTTCCAACAAGACCCGTA	52	130
	RV	AGCAGGATACGCCTTTGAGT	52
FBXL5	FW	AAGGTGCTCCAAATAAGGCA	50	119
	RV	CTGATCAAGAGACTGGACGTG	54