Table 1: List of qRT-PCR and sequencing primers of target and reference genes.
GENE | Direction | Sequence (5'-3') | Tm | Amplicon size (bp) |
Real-time qRT-PCR primers | ||||
House-keeping genes | ||||
18SrRNA | FW | GGCGCCCCCTCGATGCTCTTAG | 62 | 89 |
RV | GCTCGGGCCTGCTTTGAACACTCT | 61 | ||
28SrRNA | FW | TGTCGGCTCTTCCTATCATTGT | 53 | 81 |
RV | ACCCAGCTCACGTTCCCTATTA | 55 | ||
ACTB | FW | GTTGTCGACGACGAGCG | 52 | 93 |
RV | GCACAGAGCCTCGCCTT | 52 | ||
GAPDH | FW | TTGAGGTCAATGAAGGGGTC | 52 | 117 |
RV | GAAGGTGAAGGTCGGAGTCA | 54 | ||
Target genes | ||||
DMT1-NIRE | FW | TCCCCATGATCTCCAGAAAC | 52 | 115 |
RV | GGTTGCGGAGCTGGTAAG | 53 | ||
DMT1-IRE | FW | TGCACCATGAGGAAGAAGCA | 52 | 134 |
RV | ACACTGTCATCTGACATCTTCTGT | 57 | ||
TFR2 | FW | AATGTCCTGAGTCAGAGCGG | 56 | 141 |
RV | GGCAGACTCTACTGGAGCGA | 56 | ||
FPN | FW | ATGGGGGCTAAGATGTTGGT | 52 | 108 |
RV | CCAAAGGGATTGGATTGTTG | 50 | ||
FTH | FW | TGGTTCTGCAGCTTCATCAG | 52 | 148 |
RV | TGGAGCTCTACGCCTCCTAC | 56 | 56 | |
FTL | FW | GCCTGGTTCAGCTTTTTCTC | 52 | 145 |
RV | GCTACGAGCGTCTCCTGAAG | 56 | ||
IRP1 | FW | CAATGGCTCAGCAAGGTGT | 51 | 108 |
RV | CTGCTTGGGTCAGGTTCG | 53 | ||
IRP2 | FW | AGCTTCCAACAAGACCCGTA | 52 | 130 |
RV | AGCAGGATACGCCTTTGAGT | 52 | ||
FBXL5 | FW | AAGGTGCTCCAAATAAGGCA | 50 | 119 |
RV | CTGATCAAGAGACTGGACGTG | 54 |