Medium/Reagent

Components

Proliferation

·         10% FBS (#F7524, Sigma-Aldrich)

·         1% PS (#P4333, Sigma-Aldrich)

·         In DMEM/F12incl. Glutamax (#, Thermo Fisher)

Differentation

·         0.25% Glutamine (G#7513, Sigma-Aldrich)

·         1% PS (#P4333, Sigma-Aldrich)

·         2% Neuromi × 2a plus retinoic acid (#17504044, Thermo Fisher)

·         In Neurobasal medium (#10888022, Thermo Fisher)

Progesterone (#P8783; Sigma Aldrich)

1/10/100 nM diluted in EtOH

Testosterone (#8500-1g, Sigma-Aldrich)

1/10/100 nM diluted in EtOH

AG205 (#A1487, Sigma-Aldrich)

5 nM diluted in DMSO