Table 3: Flow conditions for HF5 analyses.
| Start time (min) | End time (min) | Duration (min) | Mode | Vc (ml/min) | Vx start (ml/min) | Vx start (ml/min) |
| 0 | 1 | 1 | elution | 1 | 0 | 0 |
| 1 | 2 | 1 | focus | 1 | 0.5 | 0.5 |
| 2 | 7 | 5 | Focus + inject | 1 | 0.5 | 0.5 |
| 7 | 19 | 12 | elution | 1 | 0.65 | 0.65 |
| 19 | 20 | 1 | Elution | 1 | 0.65 | 0.05 |
| 20 | 25 | 5 | Elution + inject | 1 | 0.05 | 0.05 |
| 25 | 28 | 3 | Elution | 1 | 0 | 0 |
| 28 | 30 | 2 | Elution + inject | 1 | 0 | 0 |