Role of Caspase-11 Non-Canonical Inflammasome in Macrophage-Mediated Inflammatory Responses

Inflammation is a complex biological response mediated by macrophages to protect the body from the pathogens and danger signals. The inflammatory response is initiated by priming, a process increasing the expression of inflammatory genes by extracellular pattern-recognition receptor (PRR)mediated detection of pathogens, followed by triggering, a process detecting cytosolic pathogens by intracellular PRRs. Triggering induces the formation of intracellular PRR complexes called inflammasomes composed of two main groups; canonical and non-canonical inflammasomes. Unlike canonical inflammasomes, non-canonical inflammasomes were recently discovered, and the knowledge of the roles of non-canonical inflammasomes in inflammatory responses and human diseases is not still enough. Mouse caspase-11 and human caspase-4/5 were identified as non-canonical inflammasomes, and many efforts have been made to demonstrate the regulatory functions of these non-canonical inflammasomes in inflammatory responses and several human diseases. This review discusses the recent research progress to understand the roles of the caspase-11 non-canonical inflammasome in macrophagemediated inflammatory responses, which can provide the insight and contribute to developing potential diagnostic and therapeutic agents to prevent and treat human infectious and inflammatory/autoimmune diseases.

Canonical inflammasomes have long been investigated in inflammatory conditions and several human diseases, by contrast, non-canonical inflammasomes, such as mouse caspase-11 and human caspase-4/5 were recently discovered.However, although the knowledge of the functional roles of these non-canonical inflammasomes is limited, a number of emerging studies have successfully demonstrated the regulatory roles of caspase-11 non-canonical inflammasomes in inflammatory responses and human diseases.This review aimed to summarize and discuss the recent study progress in understanding the regulatory roles of the caspase-11 non-canonical inflammasome in macrophage-mediated inflammatory responses and to provide the insight for the development of promising drugs to prevent and treat various infectious and inflammatory/autoimmune diseases.

Structure and Activation of Inflammasomes
Inflammasomes are classified into two groups; canonical and non-canonical inflammasomes.There are several types of canonical inflammasomes, such as NLR family inflammasomes, including NLRP1, NLRP3, and NLRC4 and non-NLR family inflammasome, including The structure of canonical and non-canonical inflammasomes A) NLRP1 consisting of a PYD, a NACHT, LRRs, a FIIND, and a CARD assembles inflammasome by the interaction of NLRP1 with ASC through their PYDs and the subsequent interaction with pro-caspase-1 through their CARDs; B) NLRP3 consisting of a PYD, a NACHT, and LRRs assembles inflammasome by the interaction of NLRP3 with ASC through their PYDs and the subsequent interaction with pro-caspase-1 through their CARDs; C) NLRC4 consisting of a CARD, a NACHT, and LRRs assembles inflammasome by the direct interaction of NLRC4 and pro-caspase-1 through their CARDs; D) AIM2 consisting of a PYD and a HIN200 domain assembles inflammasome by the interaction of AIM2 and ASC through their PYDs and subsequent interaction with pro-caspase-1 through their CARDs; E) Mouse caspase-11 and human caspase-4/5 consisting of a CARD, a p20, and a p10 assemble inflammasomes by the direct interaction of caspase-4/5/11 CARDs and the lipid A moiety of intracellular LPS; F) Once caspase-4/5/11 bind with intracellular LPS, LPS-caspase-4/5/11 complexes are oligomerized and activated.NLR: Nucleotide-binding oligomerization domain-like receptor; PYD: Pyrin domain; NACHT: Nucleotide-binding and oligomerization domain; LRRs: Leucine-rich repeats; FIIND: Function to find domain; CARD: Caspase recruit domain; Caspase: Cysteine-aspartic protease; AIM2: Absent in melanoma 2; HIN: Hematopoietic interferon-inducible nuclear proteins; Caspase: Cysteine-aspartic protease; LPS: Lipopolysaccharide.* : Autocatalytic cleavage site.

Role of Caspase-11 Non-canonical Inflammasome in Macrophage-mediated Inflammatory Responses
Previous studies have successfully demonstrated that inflammasomes are activated by a variety of ligands, present in innate immune cells, such as M1 macrophages during inflammatory responses.Interestingly, unexpected observation was reported that cholera toxin B-induced inflammasome activation and inflammatory responses were abolished in the bone marrow-derived macrophages isolated from the 129S6 mouse strain which lacks functional caspase-11 proteins due to the polymorphism in the caspase-11 gene locus and that septic shock induced by the lethal dose of LPS was much less in these mice [19].This observation suggests that caspase-11 is not a member of canonical inflammasomes and plays a unique role during macrophage-mediated inflammatory responses in a canonical inflammasome-independent manner, therefore, the caspase-11 inflammasome was named as non-canonical inflammasome.
As discussed earlier, caspase-11 non-canonical inflammasome is activated by intracellular LPS derived  caspase-11 oligomers and the activation of caspase-11 non-canonical inflammasome [21,23].The activation of caspase-11 non-canonical inflammasome subsequently induces the cleavage of GSDMD at the aspartic acid 276 residue (Asp 276 ) and produces N-terminal GSDMD fragments that move to the cell membrane and generate GSDMD pores, leading to the pyroptosis [10][11][12][13].Simultaneously, the activation of caspase-11 non-canonical inflammasome also induces the activation of NLRP3 inflammasome and the proteolytic maturation of inactive pro-caspase-1 to active caspase-1, an effector of inflammasome activation, followed by the proteolytic maturation of inactive pro-inflammatory cytokines, pro-IL-1β and pro-IL-18 to active IL-1β and IL-18 by caspase-1, leading to the secretion of IL-1β and IL-18 through the GSDMD pores [10][11][12][13].Molecular mechanisms by which caspase-11 non-canonical inflammasome activates NLRP3 canonical inflammasome is still unclear, and the studies elucidating the crosstalk between these canonical and non-canonical inflammasomes in macrophage-mediated inflammatory responses need to be further investigated.The molecular events of caspase-11 non-canonical inflammasome activation during macrophage-mediated inflammatory responses discussed in this chapter are summarized in Figure 2.

Conclusions and Future Perspectives
The inflammatory response occurs by two consecutive steps; priming and triggering, and the triggering is induced by the activation of inflammasomes.Although regulatory roles and the underlying molecular mechanisms of canonical inflammasome activation in inflammatory responses and a variety of human diseases have long been studied [7,9,11,[25][26][27][28], noncanonical inflammasomes, such as mouse caspase-11 and human caspase-4/5 were recently discovered, and emerging studies have demonstrated that unlike canonical inflammasomes, caspase-11 non-canonical inflammasome is activated by direct interaction with intracellular LPS originated from gram-negative bacteria, which induces the GSDMD-mediated pyroptosis of inflamed macrophages and the activation of caspase-1, resulting in the maturation and secretion of pro-inflammatory cytokines, IL-1β and IL-18 through GSDMD pores.Despite a number of recent studies investigating the roles of caspase-11 non-canonical inflammasome in macrophage-mediated inflammatory responses, further studies demonstrating 1) The regulatory roles of caspase-11 non-canonical inflammasome in the pathogenesis of human diseases, 2) The functional crosstalk between canonical and caspase-11 noncanonical inflammasomes in inflammatory responses, 3) The missing puzzle pieces in the caspase-11 noncanonical inflammasome-activated inflammatory responses, and 4) The development of selective and efficient targeting strategies to modulate the activation from gram-negative bacteria and is not activated by the gram-positive bacteria due to the lack of LPS [20].Hager, et al. also demonstrated that caspase-11 was activated and induced inflammatory responses in the macrophages transfected with the lysates of gram-negative bacteria, but not those of gram-positive bacteria [21].These studies strongly indicate that caspase-11 non-canonical inflammasome is activated by recognizing intracellular LPS originated from gram-negative bacteria.However, despite the evidence that caspase-11 recognizes intracellular LPS to be activated, how caspase-11 interacts with intracellular LPS was still under question.Biochemical analysis using recombinant caspase-11 expressed and purified in the gram-negative bacterium, E. coli demonstrated that caspase-11 directly interacts with LPS to form a huge caspase-11 multimer [18], and unlike canonical inflammasomes, caspase-11 does not need adaptor molecules, such as ASC for the interaction with its ligand, intracellular LPS.Similar with mouse caspase-11, recombinant caspase-4/5 expressed and purified in E. coli directly interacts with E. coli-derived LPS, and the caspase-4/5 multimer was produced, while the caspase-4/5 multimer was not formed in the insect cells [18].Further studies have demonstrated that lipid A moiety of LPS is a critical determinant and sufficient for the interaction with caspase-4/5/11 and the subsequent activation of caspase-4/5/11 non-canonical inflammasomes in macrophage-mediated inflammatory responses [18,21], and that hexa-and penta-acylated lipid A can bind with caspase-4/5/11 and induce the activation of caspase-4/5/11 non-canonical inflammasomes in macrophages, while tetra-acylated lipid A cannot [21,22].These studies suggest that mouse caspase-11 and human caspase-4/5 directly recognize intracellular LPS derived from the gram-negative bacteria, leading to the activation of caspase-4/5/11 non-canonical inflammasomes in macrophage-mediated inflammatory responses.
Many studies have successfully demonstrated the inflammatory events induced by the activation of caspase-11 non-canonical inflammasome in M1 macrophages.Induction of macrophage-mediated inflammatory responses requires two main steps; priming and triggering.Priming is the process by which PRRs recognize PAMPs of pathogens, resulting in the induction of the inflammatory signaling cascades and gene expression of inflammasome components that are required for inflammasome activation [19,23,24].Priming, itself, neither activates inflammasome activation signaling nor induces the maturation and activation of the inactive-forms of inflammatory molecules, such as pro-caspase-1, pro-IL-1β and pro-IL-18, and pyroptosis, therefore, the second triggering step is required for initiation of inflammasome activation in macrophages during inflammatory responses.The triggering step is initiated through the direct recognition of intracellular LPS by caspase-11, leading to the formation of LPS-ling, including CRISPR/Cas9, small interference RNA, and cell-penetrating antibody [29] could be a promising strategies to prevent and treat a variety of human infectious and inflammatory/autoimmune diseases. of caspase-11 non-canonical inflammasome during inflammatory responses need to be investigated.
In conclusion, given the evidence from the previous studies, caspase-11 non-canonical inflammasome plays a pivotal role to induce inflammatory responses in macrophages.Furthermore, useful technologies to selectively target caspase-11 non-canonical inflammasome signa-

Figure 1 :
Figure 1:The structure of canonical and non-canonical inflammasomes A) NLRP1 consisting of a PYD, a NACHT, LRRs, a FIIND, and a CARD assembles inflammasome by the interaction of NLRP1 with ASC through their PYDs and the subsequent interaction with pro-caspase-1 through their CARDs; B) NLRP3 consisting of a PYD, a NACHT, and LRRs assembles inflammasome by the interaction of NLRP3 with ASC through their PYDs and the subsequent interaction with pro-caspase-1 through their CARDs; C) NLRC4 consisting of a CARD, a NACHT, and LRRs assembles inflammasome by the direct interaction of NLRC4 and pro-caspase-1 through their CARDs; D) AIM2 consisting of a PYD and a HIN200 domain assembles inflammasome by the interaction of AIM2 and ASC through their PYDs and subsequent interaction with pro-caspase-1 through their CARDs; E) Mouse caspase-11 and human caspase-4/5 consisting of a CARD, a p20, and a p10 assemble inflammasomes by the direct interaction of caspase-4/5/11 CARDs and the lipid A moiety of intracellular LPS; F) Once caspase-4/5/11 bind with intracellular LPS, LPS-caspase-4/5/11 complexes are oligomerized and activated.

Table 1 :
Summary of inflammasome structure and ligands.