Table 1: Effect of epinephrine on pro-inflammatory cytokine production by PBMC.
|
|
Non-stimulated |
LPS-stimulated |
HT-29-induced |
|
Epinephrine |
TNFα |
||
|
|
|||
|
0 |
0.68 ± 0.16 |
2.34 ± 0.2 |
3.26 ± 0.74 |
|
0.025 ng/ml |
0.67 ± 0.06 |
1.9 ± 0.24 |
3.00 ± 0.24 |
|
0.050 ng/ml |
0.66 ± 0.05 |
1.8 ± 0.14 |
2.98 ± 0.36 |
|
0.10 ng/ml |
0.67 ± 0.07 |
1.8 ± 0.14 |
3.16 ± 0.36 |
|
IL-1β |
|||
|
|
|||
|
0 |
1.44 ± 0.52 |
13.1 ± 1.14 |
8.52 ± 1.75 |
|
0.025 ng/ml |
1.20 ± 0.26 |
12.52 ± 1.2 |
7.99 ± 0.40 |
|
0.050 ng/ml |
1.22 ± 0.32 |
12.44 ± 1.12 |
8.32 ± 0.20 |
|
0.10 ng/ml |
1.42 ± 0.36 |
11.44 ± 0.96 |
8.27 ± 0.31 |
|
|
IL-6 |
||
|
0 |
4.73 ± 1.14 |
19.54 ± 4.40 |
14.81 ± 0.80 |
|
0.025 ng/ml |
4.92 ± 0.29 |
19.54 ± 0.80 |
14.60 ± 1.20 |
|
0.050 ng/ml |
4.83 ± 0.44 |
19.44 ± 0.39 |
14.45 ± 0.89 |
|
0.10 ng/ml |
5.60 ± 0.29* |
19.61 ± 0.69 |
15.71 ± 079 |
Non-stimulated PBMC or cells stimulated with either LPS or co-cultured with HT-29 colon cancer cell line, were incubated for 24 hrs without (0) or with epinephrine at concentrations as indicated. The level of cytokines in the supernatants was tested by ELISA. The results are expressed as Mean ± SEM of 6 experiments (4 different donors). Asterisk represents statistically significant difference from cells incubated without epinephrine (*p < 0.05).