Table 3:
Effect of induced Tregs on CTL generation with purified CD8+ Cells.
Functional characterization of expanded CD4+ Tregs in separate in vitro Mart-1 specific CTL generation assays with purified
CD8+ cells stimulated with autologous DCs pulsed with Mart-1 peptide.
| Effector cells from | Specific Lysis of Target cells at E:T | ||
| 40:1 | 20:1 | 10:1 | |
| UC 7 | 29 ± 10 | 12 ± 7 | 6 ± 3 |
| UC 7 + Treg A | 0 | ||
| UC 7 + Treg B | 22 ± 8 | ||
| UC 8 | 45 ± 14 | 29 ± 12 | 18 ± 10 |
| UC 8 + Treg A | 8 ± 3 | ||
| UC 8 + Treg B | NT | NT | NT |
| UC 9 | 33 ± 11 | 12 ± 7 | 9 ± 5 |
| UC 9 + Treg A | 0 | ||
| UC 9 + Treg B | 34 ± 9 | 20 ± 8 | 0 |
| UC 10 | 54 ± 19 | 35 ± 12 | 23 ± 6 |
| UC 10 + Treg A | 0 | ||
| UC 10 + Treg B | 48 ± 14 | 30 ± 9 | 20 ± 9 |
| UC 11 | 37 ± 12 | 22 ± 6 | 16 ± 4 |
| UC 11 + Treg A | 0 | ||
| UC 11 + Treg B | 28 ± 11 | 18 ± 6 | 12 ± 4 |
| UC 12 | 42 ± 15 | 28 ± 6 | 16 ± 6 |
| UC 12 + Treg A | 8 ± 6 | ||
| UC 12 + Treg B | 44 ± 17 | 31 ± 12 | 20 ± 9 |