Table 1: Subjects and adoptively infused cell characteristics.
|
Subject |
Age, y/sex |
Cell number infused (×106) |
Phenotype of infused cell populations |
|||||||
|
CD3-CD56+ NK cells |
CD3+Vγ9+ γδT cells |
CD3+Vγ9- αβT cells |
||||||||
|
%a |
Number (×106) |
CD56bright %b |
CD56dim %b |
%a |
Number (×106) |
%a |
Number (×106) |
|||
|
1 |
68/F |
2520 |
38.6 |
973 |
87.3 |
12.7 |
45 |
1134 |
16.4 |
413 |
|
2 |
53/M |
1272 |
64.8 |
824 |
94.1 |
5.9 |
17.1 |
218 |
18.1 |
230 |
|
3 |
48/F |
3520 |
83.1 |
2925 |
94.9 |
5.1 |
5.7 |
201 |
11.2 |
394 |
|
4 |
29/F |
856 |
81.6 |
698 |
94.5 |
5.5 |
10.9 |
93 |
7.5 |
64 |
|
5 |
37/M |
3040 |
95.9 |
2915 |
97.6 |
2.4 |
1 |
30 |
3.1 |
94 |
|
6 |
43/F |
3824 |
75.3 |
2879 |
98.4 |
1.6 |
13.4 |
512 |
11.3 |
432 |
|
7 |
40/F |
5536 |
89.1 |
4932 |
97.9 |
2.1 |
4 |
221 |
6.9 |
381 |
|
8 |
24/M |
4560 |
71.4 |
3256 |
97.3 |
2.7 |
28.5 |
1300 |
0.1 |
5 |
|
9 |
24/F |
5720 |
87.5 |
5005 |
94.4 |
5.6 |
4.1 |
235 |
8.4 |
480 |
|
Average |
3428 |
76 |
2712 |
95 |
5 |
14 |
438 |
9 |
277 |
|
%a cell populations were determined from the samples of eNK cells taken from the final cell culture by FACS as CD3-CD56+ for NK cells, CD3+Vγ9+ for γδ T cells, and CD3+Vγ9- for αβ T cells, respectively.
%b NK cell subsets were determined from the samples of eNK cells taken from the final cell culture by FACS after gating on CD3-CD56+ NK cells as higher expressed CD56 for CD56bright and lower for CD56dim, respectively.