Supplement 1: Primers used for analyses of gene mutations.
Gene sequence |
Codon |
Exon |
Primer set* |
Reaction sequence |
K-ras
|
12/13 |
2 |
5’-AAGGCCTGCTGAAAATGACTG-3’ 5’-GGTCCTGCACCAGTAATATGCA-3’ |
reverse |
|
|
|||
K-ras
|
61 |
3 |
5’-CAGACTGTGTTTCTCCCTTCTC-3’ 5’-CCCTCCCCAGTCCTCATG-3’ |
forward |
|
|
|||
BRAF
|
600 |
15 |
5’-CATAATGCTTGCTCTGATAGGAAA-3’ 5’-GATCCAGACAACTGTTCAAACTG-3’ |
forward |
|
|
|||
GNAS
|
201 |
8 |
5’-ACTGTTTCGGTTGGCTTTGGTGA-3’ 5’-AGGGACTGGGGTGAATGTCAAGA-3’ |
reverse |
|
|
|||
PTEN
|
7 |
|
5’-TGACAGTTTGACAGTTAAAGG-3’ 5’-GGATATTTCTCCCAATGAAAG -3’ |
forward |
|
|
|||
PTEN
|
8 |
|
5’-TTAAATATGTCATTTCATTTCTTTT-3’/ 5’TTGCTTTGTCAAGATCATT-3’ |
reverse |
|
|
|||
|
|
5’-GTGCAGATAATGACAAGGAATA-3’/ 5’-TCATGTTACTGCTACGTAAAC-3’ |
||
|
|
*Obtained from Sigma Life Science oligomer service (http://www.sigmaaldrich.com/life-science/custom-oligos/custom-dna.html)