Culture conditions and isolation methods used for CTC long-term culture.

Table 1: Culture conditions and isolation methods used for CTC long-term culture.

Cancer type	Culture conditions	Isolation methods
Breast cancer	2D culture condition [53]	Ficoll + FACS [53]
Breast cancer	Non adherent culture condition [26]	CTC-iChip [26]
Lung cancer	Xenotransplantation [21,27]	RosetteSep® + Ficoll + xenotransplantation [21,27]
Prostate cancer	organoid culture condition [20]	RosetteSep® + Ficoll [20]
Colon cancer	Non adherent culture condition [49]	RosetteSep® [49]
Gastric cancer	collagen-based 3D culture condition [60]	Magnetic micro beads [60]
Gastric cancer	Xenotransplantation [61]	Lymphoprep® + magnetic micro beads + xenotransplantation [61]