| Gene region | Primer sequences |
| KRAS exon 3 | F 5'-TGTGTTTCTCCCTTCTGAGGA-3' R 5'- AAGAAAGCCCTCCCCAGT-3' |
| POL E exon 10 | F 5'CCTCTGACTTGTGCTGATTGC'3 R 5'CCGGGTAGTTTCCCAAGTG'3 |
| BRAF exon 15 | F 5'- TGTTTTCCTTTACTTACTACCACCTCA-3' R 5'- CCACAAAATGGATCCAGACA-3' |
| BRAF exon 11 | F 5'-GACGGGACTCGAGTGATGAT-3' R 5'- TGTCACAATGTCACCACATTACA-3' |
| KRAS exon 2 | F 5'-CCTGCTGAAAATGACTGAATATAA-3' R 5'-TGGATCATATTCGTCCACAAAA-3' |
| KRAS exon 4 | F 5'-GACACAAAACAGGCTCAGGACT-3' R 5'-CAGATCTGTATTTATTTCAGTGTTA-3' |
| PIK3CA exon 9 | F 5'-AAGGGAAAATGACAAAGAACAG-3' R 5'-CACTTACCTGTGACTCCATAGAAA-3' |
| PIK3CA exon 20 | F 5'-GCAAGAGGCTTTGGAGTATTTC-3' R 5'-TTTTCAGTTCAATGCATGCTG-3' |