Table 1: Sample preparation protocol. Groups are: ISO (isotonic saline for compensation of dilutional effects),

FIB+ (fibrinogen), FXIII+ (factor XIII), FIB+FXIII+ (both substrates).

Temp

pH

Group

Fibrinogen 0.25 mg

(in μl loading solution)

FXIII 0.2 IE

( in μl loading solution)

0.9% Saline (μl)

37C

7.4

ISO

 

 

70

 

 

FIB+

20

 

50

 

 

FXIII+

 

20

50

 

 

FIB+FXIII+

20

20

30

32C

7.4

ISO

 

 

70

 

 

FIB+

20

 

50

 

 

FXIII+

 

20

50

 

 

FIB+FXIII+

20

20

30

32C

7.2

ISO

 

 

70

 

 

FIB+

20

 

50

 

 

FXIII+

 

20

50

 

 

FIB+FXIII+

20

20

30

32C

7.0

ISO

 

 

70

 

 

FIB+

20

 

50

 

 

FXIII+

 

20

50

 

 

FIB+FXIII+

20

20

30