Baseline characteristics, diagnosis, treatment received, surgical interventions and treatment outcomes of cases.

Table 2: Baseline characteristics, diagnosis, treatment received, surgical interventions and treatment outcomes of cases.

	Case 1	Case 2	Case 3	Case 4
Age	6 years	17 years	14 years	45 years
Gender	Male	Male	Male	Male
Date of specimen collection	30^th November, 2016	9^th January, 2019	24^th July, 2019	26^th September, 2020
Location of lesion(s)	Left upper limb/wrist	Right lower limb/knee, thigh, and leg	Right upper limb/arm and forearm	Left lower limb/leg
Lesion type	Ulcer	Ulcer	Ulcer	Ulcer
Clinical form, size, and WHO category of the lesion	Category II	Category III	Category III	Category III
Specimen collected for PCR diagnosis	Dry swabs and FNA in 1 mL phosphate buffered saline (PBS)	Dry swabs in sterile tubes	Dry swabs in sterile tubes	Dry swabs in sterile tubes
Specimen collected for f-TLC diagnosis	Swabs in O-ring vials containing 1 mL absolute ethanol, wrapped in aluminium foil	Swabs in O-ring vials containing 1 mL absolute ethanol, wrapped in aluminium foil	Swabs in O-ring vials containing 1 mL absolute ethanol, wrapped in aluminium foil	Swabs in O-ring vials containing 1 mL absolute ethanol, wrapped in aluminium foil
Date of laboratory diagnosis	December, 2016	January, 2019	July, 2019	June, 2020
PCR results	Positive	Negative	Positive	Positive
f-TLC results	Positive	Positive	Positive	Positive
Drug regimen • Principal drug • Secondary drug	Rifampicin (10 mg/kg) streptomycin injection (15 mg/kg)	Rifampicin (10 mg/kg) streptomycin injection (15 mg/kg)	Rifampicin (10 mg/kg) streptomycin injection (15 mg/kg)	Rifampicin (10 mg/kg) streptomycin injection (15 mg/kg)
Duration of drug therapy	8 weeks	8 weeks	8 weeks	8 weeks
Surgical treatment	No	No	Yes	No
Types of surgical Interventions	N/A	N/A	• Debridement • Skin graft	N/A
Treatment outcomes	• Antibiotic treatment completed. • Healed without surgery. • Healed without limitation of movement to any joint.	• Antibiotic treatment completed. • Healed without surgery. • Healed with limitation of movement to any joint.	• Antibiotic treatment completed. • Healed with surgery. • Healed with limitation of movement to any joint.	• Antibiotic treatment completed. • Healed without surgery. • Healed without limitation of movement to any joint.
Date of laboratory diagnosis	30^th November, 2016	9^th January, 2019	24^th July, 2019	26^th September, 2020
Date of discharge	15 March 2017	15 July, 2020	15 July, 2020	15 July, 2020
Length of hospital stay	105 days	553 days	357 days	144 days