Donor surplus human oocytes were transferred to adhesive slides. Oocytes were fixed with 1% paraformaldehyde in PBS. For permeabilization, cells were incubated in 0.1% Triton X-100 (Sigma) plus 0.1% sodium citrate in PBS. Cells were then incubated with 5% Bovine Serum Albumin (BSA; Sigma) to inhibit non-specific binding. Afterwards cells were incubated with a primary monoclonal antibody against human ZP1 (ZP1: sc-365435...

A 64-year-old man underwent catheter ablation for atrial fibrillation (AF) a month ago, which involved isolation of pulmonary veins with wide area circumferential ablation and posterior left atrial wall with roof and inferior line at 30-35 watts radiofrequency energy delivery. He presented initially with fever and palpitation, but subsequently collapsed with ventricular tachycardia and transient lateral ST elevation on electrocardiogram, and developed dysphasia with right arm weakness. Transthor...

Brain arteriovenous malformations (bAVMs) have an estimated prevalence of 10-18 per 100,000 persons. They consist of a nidus which connects arteries and veins in the absence of an intervening capillary bed. The absence of a capillary network allows for high-flow AV shunting that can be sufficient in some cases to increase cardiac output and cause a systolic-diastolic bruit heard over the ipsilateral cranium, eye, and/or forehead. Resulting shear stresses on the vessel walls can dilate and cause ...